Thoracic aortic disease: Identification of diagnostic circulating biomarkers using proteomics
Final Report Abstract
Diseases of the main artery (aorta) are among the most common causes of death in Western industrialized nations. However, the underlying dilation of the main artery (aneurysm) often remains asymptomatic until life-threatening complications such as acute Type A dissection occur. So far, suitable early detection methods are lacking. The aim of our project was therefore to develop a suitable blood marker for the early detection and monitoring of aortic diseases. To achieve this, we compared the concentrations of all proteins measurable in the blood by mass spectrometry and of all measurable exosomes (extracellular vesicles) in samples from 40 aortic patients with those from 40 healthy control subjects. Of a total of 1037 measured free and 1076 exosomal proteins, the concentrations of 10 free and 27 exosomal proteins were significantly different between the study groups. These differing proteins thus represent potential biomarker candidates for the diagnosis of aortic diseases. The concentrations of 5 of these proteins also correlate significantly with aortic diameter. In particular, the proteins Myeloperoxidase and S100-A8 appear to be promising biomarker candidates. Myeloperoxidase has previously been shown to play a crucial role in inflammatory processes in the development of aortic diseases. Proteins of the S100 family have also previously been associated with aortic diseases. For example, the structurally similar S100- A12 was found to be significantly elevated in aortic dissection patients. The identified biomarker candidates must be revalidated in future projects. Afterwards, they could contribute to earlier detection of aortic diseases and better estimation of the prognosis of these diseases. Thus, diagnosed patients could be timely provided with prophylactic surgical therapy, which can prevent life-threatening complications.
Publications
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Thoracic Aortic Diseases: Identification of Diagnostic Biomarkers Using Proteomic Analysis. The Thoracic and Cardiovascular Surgeon. Georg Thieme Verlag KG.
Arndt, N.; Voß, H.; Mair, T.; Schlüter, H.; Förster, L.; Knochenhauer, T.; Sachse, M.; Beyer, M.; Leonhardt, M.; Von Kodolitsch, Y.; Schlein, C.; Kubisch, C.; Reichenspurner, H.; Detter, C.; Rosenberger, G. & Demal, T.J.
