Understanding nuclear pore complexity (P17)

• Subject Area: Structural Biology
• Term: since 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 450648163

Nuclear pore complexes (NPC) bridge the nuclear membranes and mediate nucleocytoplasmic exchange. The central channel of the NPC is lined with intrinsically disordered, FG-rich nucleoporins (Nups) that interact with cargos. How exactly those FG-Nups distribute within the central channel and how they may adapt to conformational changes of the NPC remains unknown. In project P17, we propose to develop residue specific labeling methods for correlative light and electron microscopy to address this challenge. This novel method will allow to jointly measure the conformations of intrinsically disordered FG-domains and structured Nups.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1507: Protein Assemblies and Machineries in Cell Membranes
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Heads: Professor Dr. Martin Beck; Professor Dr. Edward A. Lemke