Adenine nucleotide control of dendritic cell migration (A20*)

• Subject Area: Cell Biology
• Term: since 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 335447717

We hypothesize that soluble factors derived from adipose tissue under inflammation, such as ATP and ADO, differentially control dendritic cell (DC) migration. In order to test this hypothesis we will: (i) analyze purinergic signaling during the migration of murine DCs, by using microfluidic devices; (ii) evaluate Ca2+ signaling during the DC migration, with a focus in the ER, by combining live-cell imaging and Ca2+ sensitive fluorescent probes; (iii) analyze the effect of AN-derived from adipose tissue over DC migration in different levels of complexity, 2D within microfluidics, 3D in collagen matrix, and in vivo in mouse models.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1328: Adenine Nucleotides in Immunity and Inflammation
• Applicant Institution: Universität Hamburg
• Project Head: Professor Dr. Pablo Sáez