Laminin-binding integrins and other receptors in the control of epithelial cell migration

• Antragstellerin: Professorin Dr. Monique Aumailley
• Fachliche Zuordnung: Biochemie
• Förderung: Förderung von 1998 bis 2008
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5111096

Cell migration is a complex process. It requires I) cytoskeleton integrity, ii) integrin-mediated contacts with the extracellular matrix and signal transmission at cell adhesion complexes, and III) remodelling of the latter. In the extracellular matrix, laminins are specific ligands for the a3b1, a6b1 and a6b4 integrins which induce morphologically different cell adhesion complexes. The a3b1 integrin transdominantly regulates other integrins, including a6b1, the formation of cell adhesion complexes and cell migration. It is unclear, however, how the a3b1 integrin exerts this control. Intracellular interactions with certain isoforms of PKC could be involved. Our aim is to determine whether and how specific PKC isoforms are associated with the remodelling of integrin signaling complexes during laminin-induced cell migration. This will be approached with biochemical analyses and immunocytochemistry, as well as by using cells from mouse embryos deficient in PKC isoforms after gene inactivation.

• DFG-Verfahren: Schwerpunktprogramme
• Teilprojekt zu: SPP 1049: Molekulare Steuerungsmechanismen der Zellwanderung