Enzymologie der intein-codierten homing-Endonuklease PI-SceI aus Saccharomyces cerevisiae

• Antragstellerin: Dr. Vera Pingoud (†)
• Fachliche Zuordnung: Biochemie
• Förderung: Förderung von 1999 bis 2009
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5199990

• Erstellungsjahr: 2009

Zusammenfassung der Projektergebnisse

Keine Zusammenfassung vorhanden

Projektbezogene Publikationen (Auswahl)

• Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-Scel. Nucleic Acids Res. 1996 Nov 1;24(21):4123-32
  Wende W, Grindl W, Christ F, Pingoud A, Pingoud V
  
• Structural and functional analysis of the homing endonuclease Pl-scel by limited proteolytic cleavage and molecular cloning of partial digestion products. Biochemistry. 1998 Jun 2;37(22):8233-43
  Pingoud V, Grindl W, Wende W, Thole H, Pingoud A
  
• The protein splicing domain of the homing endonuclease Pl-scel is responsible for specific DNA binding. Nucleic Acids Res. 1998 Apr 15;26(8):1857-62
  Grindl W, Wende W, Pingoud V, Pingoud A
  
• A similar active site for non-specific and specific endonucleases. Nat. Struct. Biol. 1999 Feb;6(2):112-3
  Friedhoff P, Franke I, Meiss G, Wende W, Krause KL, Pingoud A
  
• Photocross-linking of the homing endonuclease Pl-Scel to its recognition sequence. J. Biol. Chem. 1999 Apr 9;274(15):10235-43
  Pingoud V, Thole H, Christ F, Grindl W, Wende W, Pingoud A
  
• The monomeric homing endonuclease Pl-Scel has two catalytic centres for cleavage of the two strands of its DNA substrate. EMBO J. 1999 Dec 15;18(24):6908-16
  Christ F, Schoettler S, Wende W, Steuer S, Pingoud A, Pingoud V
  
• A model for the PI-ScelxDNA complex based on multiple base and phosphate backbone-specific photocross-links. J. Mol. Biol. 2000 Jul 21;300(4):867-75
  Christ F, Steuer S, Thole H, Wende W, Pingoud A, Pingoud V
  
• Analysis of binding and cleavage of DNA by the gene conversion Pl-SCEl endonuclease using sitedirected mutagenesis. Mol. Biol. (Mosk). 2000 Nov-Dec;34(6):1054-64
  Wende W, Schöttler S, Grindl W, Christ F, Steuer S, Nöel AJ, Pingoud V, Pingoud A
  
• Identification of Asp218 and Asp326 as the principal Mg2+ binding ligands of the homing endonuclease Pl-Scel. Biochemistry. 2000 Dec 26;39(51): 15895-900
  Schöttler S, Wende W, Pingoud V, Pingoud A
  
• High resolution crystal structure of domain l of the Saccharomyces cerevisiae homing endonuclease Pl-Scel. Nucleic Acids Res. 2002 Sep 15;30(18):3962-71
  Werner E, Wende W, Pingoud A, Heinemann U
  
• Homingendonukleasen - Vielversprechende Werkzeuge für das Genomengineering. BIOspektrum 2003; 9(5):592-95
  Pingoud A, Pingoud V, Wende W
  
• Chimeras of the homing endonuclease Pl-Scel and the homologous Candida tropicalis intein: a study to explore the possibility of exchanging DNA-binding modules to obtain highly specific endonucleases with altered specificity. Chembiochem. 2004 Feb 6;5(2):206-13
  Steuer S, Pingoud V, Pingoud A, Wende W
  
• DNA recognition by the homing endonuclease Pl-Scel involves a divalent metal ion cofactor-induced conformational change. J. Biol. Chem. 2004 Feb 20;279(8):6794-804
  Nöel AJ, Wende W, Pingoud A
  
• From monomeric to homodimeric endonucleases and back: engineering novel specificity of LAGLIDADG enzymes. J. Mol. Biol. 2006 Aug 25;361(4):744-54
  Silva GH, Belfort M, Wende W, Pingoud A
  
• Precision genome surgery. Nat. Biotechnol. 2007 Jul;25(7):743-4
  Pingoud A, Silva GH