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Enzymologie der intein-codierten homing-Endonuklease PI-SceI aus Saccharomyces cerevisiae

Applicant Dr. Vera Pingoud (†)
Subject Area Biochemistry
Term from 1999 to 2009
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 5199990
 
Final Report Year 2009

Final Report Abstract

No abstract available

Publications

  • Binding, bending and cleavage of DNA substrates by the homing endonuclease Pl-Scel. Nucleic Acids Res. 1996 Nov 1;24(21):4123-32
    Wende W, Grindl W, Christ F, Pingoud A, Pingoud V
  • Structural and functional analysis of the homing endonuclease Pl-scel by limited proteolytic cleavage and molecular cloning of partial digestion products. Biochemistry. 1998 Jun 2;37(22):8233-43
    Pingoud V, Grindl W, Wende W, Thole H, Pingoud A
  • The protein splicing domain of the homing endonuclease Pl-scel is responsible for specific DNA binding. Nucleic Acids Res. 1998 Apr 15;26(8):1857-62
    Grindl W, Wende W, Pingoud V, Pingoud A
  • A similar active site for non-specific and specific endonucleases. Nat. Struct. Biol. 1999 Feb;6(2):112-3
    Friedhoff P, Franke I, Meiss G, Wende W, Krause KL, Pingoud A
  • Photocross-linking of the homing endonuclease Pl-Scel to its recognition sequence. J. Biol. Chem. 1999 Apr 9;274(15):10235-43
    Pingoud V, Thole H, Christ F, Grindl W, Wende W, Pingoud A
  • The monomeric homing endonuclease Pl-Scel has two catalytic centres for cleavage of the two strands of its DNA substrate. EMBO J. 1999 Dec 15;18(24):6908-16
    Christ F, Schoettler S, Wende W, Steuer S, Pingoud A, Pingoud V
  • A model for the PI-ScelxDNA complex based on multiple base and phosphate backbone-specific photocross-links. J. Mol. Biol. 2000 Jul 21;300(4):867-75
    Christ F, Steuer S, Thole H, Wende W, Pingoud A, Pingoud V
  • Analysis of binding and cleavage of DNA by the gene conversion Pl-SCEl endonuclease using sitedirected mutagenesis. Mol. Biol. (Mosk). 2000 Nov-Dec;34(6):1054-64
    Wende W, Schöttler S, Grindl W, Christ F, Steuer S, Nöel AJ, Pingoud V, Pingoud A
  • Identification of Asp218 and Asp326 as the principal Mg2+ binding ligands of the homing endonuclease Pl-Scel. Biochemistry. 2000 Dec 26;39(51): 15895-900
    Schöttler S, Wende W, Pingoud V, Pingoud A
  • High resolution crystal structure of domain l of the Saccharomyces cerevisiae homing endonuclease Pl-Scel. Nucleic Acids Res. 2002 Sep 15;30(18):3962-71
    Werner E, Wende W, Pingoud A, Heinemann U
  • Homingendonukleasen - Vielversprechende Werkzeuge für das Genomengineering. BIOspektrum 2003; 9(5):592-95
    Pingoud A, Pingoud V, Wende W
  • Chimeras of the homing endonuclease Pl-Scel and the homologous Candida tropicalis intein: a study to explore the possibility of exchanging DNA-binding modules to obtain highly specific endonucleases with altered specificity. Chembiochem. 2004 Feb 6;5(2):206-13
    Steuer S, Pingoud V, Pingoud A, Wende W
  • DNA recognition by the homing endonuclease Pl-Scel involves a divalent metal ion cofactor-induced conformational change. J. Biol. Chem. 2004 Feb 20;279(8):6794-804
    Nöel AJ, Wende W, Pingoud A
  • From monomeric to homodimeric endonucleases and back: engineering novel specificity of LAGLIDADG enzymes. J. Mol. Biol. 2006 Aug 25;361(4):744-54
    Silva GH, Belfort M, Wende W, Pingoud A
  • Precision genome surgery. Nat. Biotechnol. 2007 Jul;25(7):743-4
    Pingoud A, Silva GH
 
 

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