Dynamics of the assembly, disassembly and molecular organization of the PII signaling machinery (B12*)

• Subject Area: Biochemistry
• Term: since 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 403222702

PII proteins form homotrimers that sense the metabolic state of the cell and dynamically associate and by this regulate various other protein complexes with crucial functions in carbon and nitrogen metabolism. We want to analyse the dynamic association of PII with two central enzymes in carbon and nitrogen metabolism using structural biology approaches. We will also investigate PII Lys-carboxylation as post-translational modification and its effect on the PII interactome.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1381: Dynamic organization of cellular protein machineries: From biogenesis and modular assembly to function
• Applicant Institution: Albert-Ludwigs-Universität Freiburg
• Project Head: Professor Dr. Khaled Selim