Molekulare Interaktionen zwischen Mikrofibrillen und Basalmembranen

• Antragsteller: Dr. Dieter Reinhardt
• Fachliche Zuordnung: Dermatologie
• Förderung: Förderung von 2000 bis 2005
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5250556

Anchorage of microfibrils to basement membranes is a universal principle of multicellular organisms. The specific subjects of this proposal focus on molecular mechanisms of microfibril-basement membrane interactions. We propose the following approaches:1. Ligand binding between microfibrillar and basement membrane proteins will be examined with highly purified proteins from either recombinant or tissue sources. 2. To detect protein interactions not covered under "1", we will develop a new surface display technology and use it to screen for ligand binding interactions between microfibrillar and basement membrane proteins.3. A model system with human lens capsules will be developed. This system allows us to test anchorage and growth of microfibrils within basement membranes.4. Electron microscopic characterization of the microfibril-basement membrane interface.From these projects, we expect important contributions for (i) the understanding of functional relationships of basement membranes with their environment, (ii) new strategies in tissue engineering (e.g. blood vessels and skin equivalents), and (iii) the development of therapeutic concepts for diseases characterized by distrubed microfibrils-basement membrane interfaces.

• DFG-Verfahren: Schwerpunktprogramme
• Teilprojekt zu: SPP 1086: Genetische und molekulare Analyse von Basalmembranen und Basalmembranverankerung
• Beteiligte Person: Professor Dr. Thilo Wedel