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Projekt
Structure of modules of the respiratory NADH:ubiquinone oxidoreductase (complex I)
Antragsteller
Professor Dr. Thorsten Friedrich
Fachliche Zuordnung
Stoffwechselphysiologie, Biochemie und Genetik der Mikroorganismen
Förderung
Förderung von 2001 bis 2007
Projektkennung
Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5307268
The structure and function of the proton-pumping NADH:ubiquinone oxidoreductase, also called respiratory complex I, is poorly understood due to its complex composition. A minimal form of the complex made in bacteria is constructed of a hydrophilic NADH dehydrogenase module, an amphipatic connecting module and a hydrophobic membrane module. We are trying to crystallize the complex and its modules from Escherichia coli and from the hyperthermophilic bacterium Aquifex aeolicus. The overall structure of the E. coli complex I from E coli will be also determined by electroncryomicroscopy of two-dimensional membranes of complex I and the membrane module.
DFG-Verfahren
Schwerpunktprogramme
