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Projekt
Dynamic assembly of localized mRNP complexes in yeast
Antragsteller
Professor Dr. Ralf-Peter Jansen
Fachliche Zuordnung
Zellbiologie
Förderung
Förderung von 2001 bis 2008
Projektkennung
Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5468076
Intracellular localisation of messenger RNAs is a widespread mechanism to achieve asymmetric protein distribution. A number of localised mRNAs as well as the nature of their corresponding localisation signals are known. However, little is understood about the proteins that assemble with the mRNA during different stages of localisation or about the dynamic reorganisation of localised mRNA protein-complexes (mRNPs) during nuclear export, cytoplasmic transport and anchoring at the target site. In budding yeast, the mRNA encoding the transcription factor Ash1p is localised during anaphase to one of the two cells emerging from mitosis. Cytoplasmic transport of the mRNA requires a molecular motor, Myo4p, as well as adapter proteins that connect the RNA and the motor. We would like to understand when and how these proteins assemble with ASH1 mRNA and what other predicted but still unknown factors control the ASH1 RNP's nuclear export, cytoplamic localisation and anchoring at the destination site.
DFG-Verfahren
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