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Projekt Druckansicht

Chemische und zellbiologische Steuerung der in vitro-Remodellierung von Hydroxylapatit

Fachliche Zuordnung Biomaterialien
Förderung Förderung von 2001 bis 2008
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5315376
 
Erstellungsjahr 2013

Zusammenfassung der Projektergebnisse

Within the priority program a co-culture model, consisting of an artificial extracellular bone matrix (mineralized collagen), osteoblasts and osteoclasts could be developed which allows insights in biomineralization phenomena and remodeling mechanisms of mineralized tissue. The effects of osteocalcin and phosphoserine (the latter as a model for the non-colleagenous protein osteopontin) on collagen fibril reassembly, mineralization – and also cellular behavior were studied using this system. The formation of osteoclast-like cells from human monocytes depends very much on the kind of substrate they are attached to. The ratio of multinucleated to mononuclear cells differs between glass, plastic and hydroxyapatite containing materials. Addition of phosphoserine, the mineral-binding amino acid in the bone matrix protein osteopontin, leads to a changed microstructure of hydroxyapatite materials, absence of multinucleated cells and to a decrease in transcription levels of osteoclastic genes such as TRAP and cathepsin K. In contrast, osteocalcin, that also changes microstructure, does not affect transcription levels in the tested concentrations.

Projektbezogene Publikationen (Auswahl)

 
 

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