Biochemical and functional analysis of laminin isoforms, laminin-5, -6 and -7

• Antragstellerin: Dr. Takako Sasaki
• Fachliche Zuordnung: Dermatologie
• Förderung: Förderung von 2002 bis 2006
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5393518

The proposed project plans the precise molecualr analysis of structure-function relationships of the basement membrane protein nidogen-2. Individual domains of the protein will be produced recombinantly and serve for the mapping of binding sites for protein ligands (laminin, perlecan, etc.) and cell adhesion. These epitopes will be additionally characterized by site directed mutagenesis and X-ray crystallography of individual domains and their complexes with ligands. Further studies will be performed to characterize some autosomal dominant mutants of human nidogen-2 which could be associated with a vascular phenotype.

• DFG-Verfahren: Schwerpunktprogramme
• Teilprojekt zu: SPP 1086: Genetische und molekulare Analyse von Basalmembranen und Basalmembranverankerung