Protein-to-DNA electron transfer reactions using DNA binding enzymes

• Antragstellerin: Dr. Alexandra Spieß
• Fachliche Zuordnung: Organische Molekülchemie - Synthese, Charakterisierung
• Förderung: Förderung von 2004 bis 2008
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5429976

Recently electron transfer reactions can be observed between peptides or proteines and the DNA. Lys-Trp-Lys and Lys-Tyr-Lys have been as model peptides and M.Hhal was used as first example of a DNA-binding protein. The project of this research fellowship will be the investigation of DNA-mediated electron-transfer reactions modulated by the Fe-S-cluster containing enyzmes MutY, Endo III or SoxR. It is planned to observe the electron transfer process spectroscopically and analyze the resulting oxidative DNA damage by gel electrophoresis.

• DFG-Verfahren: Forschungsstipendien