Characterization of matrix proteins of the coralline demosponge Astrosclera willeyana, - A contribution to understanding the evolution of biomineralization

• Antragsteller: Professor Dr. Gert Wörheide
• Fachliche Zuordnung: Paläontologie
• Förderung: Förderung von 2005 bis 2010
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5443799

Biomineralization refers to the processes by which organisms forms minerals under biological control and organic macromolecules ("organic matrix") are almost always involved in controlling mineralization. Structures formed are often integral and functional parts of the organisms, such as bones, shells, and teeth, some of which show exceptional strength and beauty, often of high commercial value... In the geological record, major animal phyla began biominieralizing in a relatively short time interval in the late Precambrian - early Cambrian, about 550-520 Myrs ago, but it is still unknown whether the formation of biominerals is a homologous process with an underlying conserved physiological machinery of evolved several times independently. In this project we aim to investigate intracrystalline matrices involved in the biocalcification process of the most ancestral calcifying metazoans, coralline demosponges, using the stromatoporoid-grade Astrosclera 'willeyana' as a model species. We will first isolate constituent macromolecules by cromatographic or preparative fractionation, and subsequently carry out structural-, functional- and evolutionary analyses involving, among others, immunological/histological methods, molecular cloning polymerase chain reaction experiments. We will also utilize a recently constructed cDNA libary to screen for matrix-protein coding genes. Results of this project will provide an evolutionary road map to follow diversification of metazoan biocalcification.

• DFG-Verfahren: Sachbeihilfen
• Beteiligte Person: Professor Dr. Joachim Reitner