SFB 638:  Dynamics of Macromolecular Complexes in Biosynthetic Transport

• Subject Area: Biology
• Term: from 2004 to 2015
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5485988

The Collaborative Research Centre aims at analysing the dynamics of complex multiprotein systems that cause folding, intracellular transport and specific localisation of macromolecules. Dynamics are of interest i) at a cell biological level to describe the formation of higher complexes by protein/protein, protein/nucleic acid, and protein lipid interactions central for all projects of the program, and ii) with respect to the kinetics of changes within such individual complexes, e.g. their conformational change, polymerisation or formation of subcomplexes. This aspect will be analysed by biochemical/biophysical methods in some of the projects. A complementation and extension of topics is reached specifically by combining virological and molecular cell biological projects within a mutual initiative. Synergetic effects will arise from similar concepts and questions asked in these fields of biological research.

• DFG Programme: Collaborative Research Centres

Completed projects

• A01 - Mechanism of action of ribosome-associated chaperones in co-translational folding and transport of proteins (Project Heads Bukau, Bernd ;  Kramer, Günter )
• A02 - Signal peptides and the insertion of TA proteins into te membrane of the endoplasmic reticulum (Project Head Dobberstein, Bernhard )
• A03 - Structure and Dynamics of Protein/RNA complexes in SRP-mediated protein transport (Project Heads Sinning, Irmgard ;  Wild, Klemens )
• A04 - Mechanismus und Regulation der ER-Lokalisation durch Arginin-Signale (Project Head Schwappach-Pignataro, Blanche )
• A05 - Biogenesis, Structure and Dynamics of flaviviral replication complexes (Project Head Bartenschlager, Ralf Friedrich Wilhelm )
• A07 - Biogenese von Peroxisomen (Project Head Just, Wilhelm )
• A08 - Unconventional Protein Secretion (Project Head Nickel, Walter )
• A09 - Formation and dynamic alterations of retroviral transport, assembly and budding complexes (Project Head Kräusslich, Hans-Georg )
• A10 - Dynamics of COPI Assembly (Project Heads Brügger, Britta ;  Wieland, Felix Wilhelm Theodor )
• A11 - Modulation of architecture and function of the immunological synapse by the HIV pathogenesis factor Nef (Project Head Fackler, Ph.D., Oliver T. )
• A13 - Mechanism and dynamics of Hsp70 and Hsp90-assisted folding of Transcription factors and kinases (Project Head Mayer, Matthias Peter )
• A15 - Koordination und Regulation der homotypischen Fusion von Hefevakuolen; Mechanismus und Funktion der Palmitoylierung von Fusionsfaktoren (Project Head Ungermann, Christian )
• A16 - Structural dynamics of coatomer (Project Heads Briggs, John ;  Wieland, Felix Wilhelm Theodor )
• A18 - Structure and dynamics of the protein O-mannosylation machinery (Project Head Strahl, Sabine )
• A19 - Structure and dynamics of protein complexes in tail-anchored membrane protein biogenesis (Project Head Sinning, Irmgard )
• B01 - Transportprozesse zwischen Zellkern und Zytoplasma (Project Head Görlich, Dirk )
• B02 - Structure, Function and Reconstitution of the Nuclear Pore Complex (Project Heads Flemming, Dirk ;  Hurt, Eduard Christian )
• B03 - Formation, Maturation and Nuclear Export of mRNPs (Project Head Hurt, Eduard Christian )
• B04 - Temporal and spatial dynamics of the circadian clock of Neurospora (Project Head Brunner, Michael )
• B05 - Regulation of complexes involved in spindle positioning (Project Head Liakopoulos, Ph.D., Dimitris )
• B07 - Nuclear envelope insertion of the yeast centrosome (Project Head Schiebel, Elmar )
• B08 - Sumoylation of nuclear transport receptors (Project Head Melchior, Frauke )
• B09 - Coupling of RNA quality control to nuclear export (Project Head Fischer, Tamás )
• NW01 - Regulation of complexes involved in spindle positioning (Project Head Liakopoulos, Ph.D., Dimitris )
• Z01 - Protein analysis by mass spectrometry (Project Heads Lechner, Johannes ;  Ruppert, Thomas )
• Z02 - Central tasks of the Collaborative Research Centre (Project Head Wieland, Felix Wilhelm Theodor )
• Z03 - Flow Cytometry/Fluorescence Activated Cell Sorting (FACS) (Project Heads Knop, Michael ;  Sourjik, Victor )
• Z04 - HTP-Protein crystallization platform and X-ray structure determination (Project Head Sinning, Irmgard )

• Applicant Institution: Ruprecht-Karls-Universität Heidelberg
• Participating Institution: European Molecular Biology Laboratory (EMBL)
• Spokesperson: Professor Dr. Felix Wilhelm Theodor Wieland