Molecular and structural analysis of plant lipid flippases

• Applicant: Professor Dr. Thomas Günther-Pomorski
• Subject Area: Biochemistry
• Term: since 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 549962795

P4-ATPases are a major family of lipid flippases found in all eukaryotic cells. They play a critical role in essential processes such as vesicle formation within the secretory/endocytic pathway. The mechanisms underlying lipid recognition and regulation of P4-ATPases are poorly understood. This project aims to conduct a comprehensive molecular and structural investigation of plant P4-ATPases using a multifaceted approach that integrates advanced biochemical and biophysical techniques. Focusing on members of the ALA9-ALA12 subfamily of plant lipid flippases, the project will address the structural determinants that determine their substrate specificity and regulatory mechanisms. Potential autoinhibition and the effect of kinase phosphorylation on their activity will be elucidated using deletion mutations and biochemical assays. In addition, given the evidence from previous studies on the influence of phosphoinositides on P4-ATPases, the project will investigate whether these transporters are also subject to phosphoinositide regulation. By addressing these key questions, this project aims to deepen our understanding of P4-ATPase function and regulation, thereby shedding light on fundamental cellular processes and potentially guiding future biotechnological efforts.

• DFG Programme: Research Grants