Project Details
Projekt
Z1 Platform for quantitative proteomics and data integration
Subject Area
Cell Biology
Term
since 2025
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 531902955
Proteomics is a powerful, unbiased analytical tool that can detect changes in an organism’s proteome and provide an overview of the overall physiological state of a specific cell type or intact organs. The goal of the proposed Research Unit is to characterize the non-autonomous cellular signaling pathways that orchestrate inter-tissue proteostasis networks at the organismal level. Moreover, these proteomic studies are essential to understand the basic cellular processes that occur in healthy tissues and disease states. This service project will be dedicated to providing support for quantitative expression proteomics of both in vitro and in vivo systems. The objectives include comprehensive label-free quantification, enrichment for posttranslational modifications (PTMs), and interactome studies to characterize the pathways of proteostasis. The platform will also assist in the assessment of subcellular fractions, aggregates, and organelle-specific enrichment. In addition, the platform will provide tailored training to the consortium to enhance proficiency in bioinformatics analysis and data handling. The service project will advise scientists of the Research Unit in experimental design, perform standardized statistical analysis, and help to interpret the resulting proteomics data. The unit is already equipped with modern quadrupole-orbitrap and ion mobility mass spectrometers. All instruments are coupled to fast liquid chromatography systems, which allow short LC-MS gradients and enable analysis of large-scale projects via modern mass spectrometry (MS) techniques. Overall, the major focus is the application of quantitative proteomics to cultured cells and model organisms (including worms and mice) under normal and disease-related conditions.
DFG Programme
Research Units
