Project Details
Projekt
LC-MS System Triple-Quadrupol-Mas spectrometer
Subject Area
Plant Sciences
Term
Funded in 2025
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 560758240
A device is being requested for high-performance liquid chromatography-mass spectrometry analysis (HPLC-MS/MS, Tripple Quad). Complex extracts of different organisms will be separated using HPLC, and then the identity and quantity of different metabolites will be determined using the attached MS system. The primary ionization method for the MS analysis will be to be electrospray ionization (ESI). With the aim of accurately and sensitively determining a wide range of known and unknown metabolites and substances, a triple quadrupole MS is being requested. The device will be mainly used to analyze primary metabolites (e.g. amino acids and sugar phosphates) and specialized metabolites (e.g. flavonoids) in phototrophic organisms. In addition, quantification of metabolic processes and intermediates in animals and biotechnologically relevant microorganisms is sought. Additionally, the LC-MS device is intended to be used to analyze complex mixtures from the field of primary and secondary (aged) aerosols and their interaction with biological systems. Furthermore, work is planned in this context for the chemical characterization of materials from the field of energy transition (bio-oils, char, and coke materials) and circular economy (composite recycling). The departments involved in the application are part of the Interdisciplinary Faculty (INF) of the University of Rostock. The device will be included in the "Competence Center Mass Spectrometry" of the INF of the University of Rostock. As an extension of the MS center, the device will also be available to intra- and extra-university institutions. The joint use of the requested device by departments of the Institute of Biosciences and the Institute of Chemistry is suitable for promoting interdisciplinary research and initiating joint research projects, even beyond the two institutes. In addition, the device represents a desirable extension in the molecular biological and metabolic physiological training of students and doctoral candidates. Thus, the requested device promotes molecular biological sciences at the University of Rostock and supports and creates the basis for important research projects in metabolic physiology.
DFG Programme
Major Research Instrumentation
Major Instrumentation
LC-MS System Triple-Quadrupol-Massenspektrometer
Instrumentation Group
1700 Massenspektrometer
Applicant Institution
Universität Rostock
