Super-Resolution-Live-Cell-Imaging Microscope with Lattice SIM, SMLM and 3D-PALM

• Subject Area: Neurosciences
• Term: Funded in 2025
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 563245759

The Faculty for Veterinary Medicine at the Ludwig-Maximilians-Universität München is at the forefront in investigating different zoonotic diseases within the Institute of Infection Medicine and Zoonotic diseases. Research projects are deliberately diverse yet synergistic to cover a wide scientific and technological to respond to the demands in research and control of infectious disease threats in our society. The individual working groups of the institute work synergistically, and the establishment of "core facilities" such as a "single cell sequencing and imaging platform" are required. There are also collaborations and overlaps with other departments, such as Food Safety, Physiology, Physiological Chemistry, Molecular Animal Breeding and Biotechnology. In this context the Institute of Infection Medicine and Zoonotic diseases was able to hire three European Research Council (ERC)‐winners working on infectious diseases. Furthermore, members of the faculty coordinate EU‐funded, international PhD student training networks and are funded within the LMU-Cambridge-Strategic partnership (see: https://www.cambridge.uni-muenchen.de/index.html), to name a few. With the relocation of the Institute to the newly built "Oberschleißheim veterinary campus" we will have a nationwide unique infectious medicine center created, including an animal facility for security level 3 that is unique in Bavaria. It enables urgently needed investigations on the transmission and pathogenesis of emerging infectious diseases as well as clinically relevant ones in close cooperation with colleagues in human medicine. To meet all imaging requirements within the Department for Veterinary Sciences, we will also establish a new imaging platform, AIPVet (Advanced Imaging Platform for Veterinary Medicine), that will allow the characterization of infectious processes from tiny viral particles, to “large” eukaryotic pathogens in in vitro models as well as in tissues from infected animals. Therefore, we require a Fluorescence super-resolution microscope that will be placed at the new campus for Veterinary Medicine within the new building "Centre for Infectious diseases, MBio1". The microscope will be placed in a dedicated imaging suite placed within the BSL-2 laboratory within MBio1 and will form the core of AIPVet at our faculty.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: Super-Resolution-Live-Cell-Imaging Microscope mit Lattice SIM, SMLM und 3D-PALM
• Instrumentation Group: 5040 Spezielle Mikroskope (außer 500-503)
• Applicant Institution: Ludwig-Maximilians-Universität München
• Leader: Professor Dr. Markus Meißner