Localization of proteins that mediate the production of macrocyclic trichothecenes in Stachybotrys chartarum

• Applicant: Professor Dr. Sebastian Ulrich
• Subject Area: Veterinary Medical Science
• Term: since 2025
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 565924569

The project aims to better understand the biosynthesis of highly toxic mycotoxins (macrocyclic trichothecenes, MCT), which pose a significant health threat to both humans and animals. The focus is on studying the genes and proteins that are responsible for MCT production, as well as their function and spatial distribution within the fungal cells. Specifically, the project has two main objectives: 1. Localization of the biosynthesis proteins: Using fluorescent labels (GFP/RFP), proteins derived from the relevant genes will be analyzed in the fungi Aspergillus nidulans and Stachybotrys chartarum. This occurs under conditions that promote or suppress MCT production. 2. Gene deletion in S. chartarum: The targeted removal of selected genes (tri and sat genes) is intended to investigate which of these genes are essential for MCT biosynthesis. The overall goal is to decipher the genetic basis of MCT biosynthesis and the underlying metabolic pathways in S. chartarum. In the long term, this knowledge should contribute to a better understanding of the genetic regulation mechanisms of mycotoxin production and potentially to develop new approaches to risk reduction. Given the health concerns associated with moisture-damaged indoor environments, particularly after natural disasters, research on S. chartarum is of great importance. This project forms an important basis for future studies to better assess and minimize the risks posed by mycotoxins.

• DFG Programme: Research Grants