Project Details
Projekt
Microfluidic workstation for petide arrays
Subject Area
Medicine
Term
Funded in 2025
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 566187929
The human kinome consists of approximately 500 members and is responsible for modifying nearly one-third of the human proteome, clearly highlighting the enormous role kinases play in human physiology and pathology. Although several –omics approaches have been widely used over the last years, all of these focus on the cellular and function response, rather than on the initial trigger that causes this response. Upon receptor activation by ligand binding, the first response is the initiation of intracellular signalling cascades by the activation of various kinases. These kinases convey the necessary signals towards the nucleus to induce transcription and subsequent translation, resulting in a cellular response. Therefore kinomic approaches are highly valuable to elucidate the intracellular signalling pathways that lead to these cellular responses. A kinomics platform can elucidate the pathways which are involved in these intracellular signalling cascades, but can also be used for novel target identification and/or biomarker identification and therefore has a broad range of applications. Based on the described research projects and research questions, the kinomics platform has to be characterized by the following technical features: a. High coverage: The platform should cover a large amount of the kinome in order to provide valuable data. Ideally, a coverage of at least 50-60% should be reached in order to perform proper kinomics. b. Low sample input: The platform should be able to give proper results with low amounts of input, ideally less 20μg of protein. c. High throughput/low run time: The platform should be able to process a relatively large amount of samples in a short amount of time. d. Measurement of functional kinase activity: The platform should be able to measure the active function of kinases. A kinomics platform, as requested in this application, is highly relevant for many ongoing research groups at the RWTH Medical Faculty, e.g. within the SFB/TRR219 as well as the CRC1328 as well as planned SFB initiatives of our faculty. Therefore, a total of 17 project leaders from 10 institutes are participating in this application. The main applicants´ working group will operate the requested kinomics platform as a central platform to ensure standardized operating procedures. After a detailed discussion of the specific scientific question, each researcher will furnish his/her samples of interest and members of the applicants’ group will be responsible for both accumulating the kinomic data and analyzing the resulting data. This will ensure a standardized quality check and uniform sample handling.
DFG Programme
Major Research Instrumentation
Major Instrumentation
Mikrofluidische Plattform für Peptidarrays
Instrumentation Group
3190 Sonstige Geräte der Klinischen Chemie und Molekularbiologie
Applicant Institution
Rheinisch-Westfälische Technische Hochschule Aachen
