STED for a confocal-laser-scanning-microscope

• Subject Area: Basic Research in Biology and Medicine
• Term: Funded in 2025
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 567856197

Light microscopy is an indispensable tool in modern cell biology. Paired with new advances in genetically marking proteins, organelles, and other structures for imaging, ist non-perturbing nature of light make it especially suited for imaging biology in living cells. Modern detector systems support imaging for long periods of time to follow in vivo dynamics of sub-cellular structures. However, the diffraction limit of light prevents the determination and analysis of higher-order within these subcellular structures at high resolution. Our current research on non-membrane enclosed biomolecular condensates, membranous organelles and membrane-associated macromolecular assemblies in living bacteria, animals, and plants, lacks super-resolution capabilities to solve emerging questions of the numerous confocal laser scanning microscope (CLSM) users at the Biologicum and the larger biomolecular/-medical research community at the Weinberg campus as a whole. However, this state-of-the art technology has basically become standard in the research fields of local main CLSM users, such as RNA granule biology, stromule-nuclear connections, internal mitochondrial protein complex dynamics, endosome-associated pathologie

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: STED für ein Konfokales-Laser-Scanning-Mikroskop
• Instrumentation Group: 5090 Spezialmikroskope
• Applicant Institution: Martin-Luther-Universität Halle-Wittenberg
• Leader: Professor Dr. Christian R. Eckmann