Development of high-yield in vitro and in vivo protein expression systems based on Leishmania tarentolae

• Applicant: Dr. Kirill Alexandrov
• Subject Area: Biochemistry
• Term: from 2008 to 2011
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 62297779

• Final Report Year: 2009

Final Report Abstract

No abstract available

Publications

• Construction and analysis of Leishmania tarentolae transgenic strains free of selection markers. Mol Biochem Parasitol. 2007 155(2):71-83
  Mureev S, Kushnir S, Kolesnikov A, Breitling R, Alexandrov K
  
• Synthesis of a fluorescent analogue of geranylgeranyl pyprophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nature Protocols (2007) 2(11):2704-11
  Wu Y, Alexandrov K and Brunsveld L
  
• "Inject-mix-react-separate-and-quantitate" (IMReSQ) method for screening enzyme inhibitors. J Am Chem Soc. 2008 Sep 10;130(36):11862-3
  Wong E, Okhonin V, Berezovski MV, Nozaki T, Waldmann H, Alexandrov K, Krylov SN
  
• Development of selective RabGGTase inhibitors and crystal structure of a RabGGTase-inhibitor complex. Angew Chem Int Ed Engl. 2008;47(20):3747-50
  Guo Z, Wu YW, Tan KT, Bon RS, Guiu-Rozas E, Delon C, Nguyen TU, Wetzel S, Arndt S, Goody RS, Blankenfeldt W, Alexandrov K, Waldmann H
  
• Structures of RabGGTase-substrate/product complexes provide insights into the evolution of protein prenylation. EMBO J. 2008 Sep 17;27(18):2444-56
  Guo Z, Wu YW, Das D, Delon C, Cramer J, Yu S, Thuns S, Lupilova N, Waldmann H, Brunsveld L, Goody RS, Alexandrov K, Blankenfeldt W
  
• A cost-effective amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae. J. Biomol. Struct. & Dynamics. (2009)26(6):755-61
  Foldynova-Trantirkova S, Matulova J, Dotsch V, Lohr F, Cirstea I, Alexandov K, Breitling R, Lukes J, Trantirek L
  
• Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nat Chem Biol. 2009 Apr;5(4):227-35
  Nguyen UT, Guo Z, Delon C, Wu Y, Deraeve C, Fränzel B, Bon RS, Blankenfeldt W, Goody RS, Waldmann H, Wolters D, Alexandrov K
  
• Biophysical analysis of the interaction of Rab6a GTPase with its effector domains. J Biol Chem. 2009 Jan 30;284(5):2628-35
  Bergbrede T, Chuky N, Schoebel S, Blankenfeldt W, Geyer M, Fuchs E, Goody RS, Barr F, Alexandrov K
  
• Chaperone-assisted production of active human Rab8A GTPase in Escherichia coli. Protein Expr Purif. 2009 Jun;65(2):190-5
  Bleimling N, Alexandrov K, Goody R, Itzen A
  
• Farnesylation of Pex19p is Required for its Structural Integrity and Function in Peroxisome Biogenesis. J Biol Chem. 2009 284(31):20885-96
  Rucktäschel R, Thoms S, Sidorovitch V, Halbach A, Pechlivanis M, Volkmer-Engert R, Alexandrov K, Kuhlmann J, Rottensteiner H, Erdmann R
  
• Species-independent translational leaders facilitate cell-free expression. Nat Biotechnol. 2009 Aug;27(8):747-52
  Mureev S, Kovtun O, Nguyen UT, Alexandrov K
  
• Structure of the Disordered C-terminus of Rab7 GTPase Induced by Binding to the Rab Geranylgeranyl Transferase Catalytic Complex Reveals the Mechanism of Rab Prenylation. J Biol Chem. 2009 May 8;284(19): 13185-92
  Wu YW, Goody RS, Abagyan R, Alexandrov K