Entwicklungspotenzial, epigenetischer Status, Proteomprofil und Gesundheit von Mammalieroozyten und Embryonen aus Cryopreservierung und verbesserter und mehrstufiger Follikelkultur

• Antragstellerinnen / Antragsteller: Dr. Georg Josef Arnold; Professorin Dr. Ursula Eichenlaub-Ritter; Professor Dr. Georg Griesinger; Professor Dr. Thomas Haaf
• Fachliche Zuordnung: Reproduktionsmedizin, Urologie
• Förderung: Förderung von 2008 bis 2016
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 58733678

• Erstellungsjahr: 2017

Zusammenfassung der Projektergebnisse

Our studies provided major insight into safety and improvements in oocyte in vitro maturation, hormonal stimulation and follicle culture and cryopreservation and risk factors exposing oocytes to epimutation and changes in gene expression. Identification of markers of oocyte quality and supplementary factors that may improve cryotolerance and preimplantation development should aid in further improvement in oocyte quality and developmental capacity in oocyte IVM in animal models and in human ART.

Projektbezogene Publikationen (Auswahl)

• (2011) Evidence that carbonyl stress by methylglyoxal exposure induces DNA damage and spindle aberrations, affects mitochondrial integrity in mammalian oocytes. Hum Reprod 26:1843-59
  Tatone C, Heizenrieder T, Di Emidio G, Treffon P, Amicarelli F, Seidel T, Eichenlaub-Ritter U
  (Siehe online unter https://doi.org/10.1093/humrep/der140)
• Limiting dilution bisulfite (pyro)sequencing reveals parent-specific methylation patterns in single early mouse embryos and bovine oocytes.
  El Hajj N, Trapphoff T, Linke M, May A, Hansmann T, Kuhtz J, Reifenberg K, Heinzmann J, Niemann H, Daser A, Eichenlaub-Ritter U, Zechner U, Haaf T
  
• (2012) Oocyte ageing and its cellular basis. Int J Dev Biol 56:841-52
  Eichenlaub-Ritter U
  (Siehe online unter https://doi.org/10.1387/ijdb.120141ue)
• (2012) Vitrification at the preantral stage transiently alters inner mitochondrial membrane potential but proteome of in vitro grown and matured mouse oocytes appears unaffected. Hum Reprod 27:1096-111
  Demant M, Trapphoff T, Fröhlich T, Arnold GJ, Eichenlaub-Ritter U
  (Siehe online unter https://doi.org/10.1093/humrep/der453)
• (2013) Chronic exposure to a low concentration of bisphenol A during follicle culture affects the epigenetic status of germinal vesicles and metaphase II oocytes. Fertil Steril 100:1758-67
  Trapphoff T, Heiligentag M, El Hajj N, Haaf T, Eichenlaub-Ritter U
  (Siehe online unter https://doi.org/10.1016/j.fertnstert.2013.08.021)
• (2013) Epigenetic disturbances in in vitro cultured gametes and embryos: implications for human assisted reproduction. Fertil Steril 99:632-641
  El Hajj N, Haaf T
  (Siehe online unter https://doi.org/10.1016/j.fertnstert.2012.12.044)
• (2013) The impact of ovarian stimulation on the expression of candidate reprogramming genes in mouse preimplantation embryos. Cytogenet Genome Res 139:71-79
  Linke M, May A, Reifenberg K, Haaf T, Zechner U
  (Siehe online unter https://doi.org/10.1159/000343755)
• (2014) Dicarbonyl stress and glyoxalases in ovarian function. Biochem Soc Trans 42:433-8
  Tatone C, Eichenlaub-Ritter U, Amicarelli F
  (Siehe online unter https://doi.org/10.1042/BST20140023)
• (2014) Effects of supra-zero storage on human ovarian cortex prior to vitrification-warming. Reprod Biomed Online 29:251-8
  Klocke S, Tappehorn C, Griesinger G
  (Siehe online unter https://doi.org/10.1016/j.rbmo.2014.03.025)
• (2014) Human in vitro oocyte maturation is not associated with increased imprinting error rates at LIT1, SNRPN, PEG3 and GTL2. Hum Reprod 29:1995-2005
  Kuhtz J, Romero S, De Vos M, Smitz J, Haaf T, Anckaert E
  (Siehe online unter https://doi.org/10.1093/humrep/deu155)
• (2014) Pre- and postovulatory aging of murine oocytes affect the transcript level and poly(A) tail length of maternal effect genes. PLoS One 9:e108907
  Dankert D, Demond H, Trapphoff T, Heiligentag M, Rademacher K, Eichenlaub-Ritter U, Horsthemke B, Grümmer R
  (Siehe online unter https://doi.org/10.1371/journal.pone.0108907)
• (2015) Bisphenol A effects on mammalian oogenesis and epigenetic integrity of oocytes: a case-study exploring risks of endocrine disrupting chemicals. BioMed Research International
  Eichenlaub-Ritter U., Pacchierotti F
  (Siehe online unter https://doi.org/10.1155/2015/698795)
• (2015) Slow-freezing versus vitrification for human ovarian tissue cryopreservation. Arch Gynecol Obstet 291:419-26
  Klocke S, Bündgen N, Köster F, Eichenlaub-Ritter U, Griesinger G
  (Siehe online unter https://doi.org/10.1007/s00404-014-3390-6)
• (2016) Improved cryotolerance and developmental potential of in vitro and in vivo matured mouse oocytes by supplementing with a glutathione donor prior to vitrification. Mol Hum Reprod 22:867-881
  Trapphoff, T., Heiligentag M., Simon J., Staubach N., Seidel, T., Otte, K., Fröhlich T., Arnold G.J., Eichenlaub-Ritter, U
  (Siehe online unter https://doi.org/10.1093/molehr/gaw059)
• (2016) Postovulatory aging affects dynamics of mRNA, expression and localization of maternal effect proteins, spindle integrity and pericentromeric proteins in mouse oocytes. Hum Reprod 31:133-149
  Trapphoff T, Heiligentag M, Dankert D, Demond H, Deutsch D, Fröhlich T, Arnold GJ, Grümmer R, Horsthemke B, Eichenlaub-Ritter U
  (Siehe online unter https://doi.org/10.1093/humrep/dev279)
• (2016) Preovulatory aging in vivo and in vitro affects maturation rates, abundance of selected proteins, histone methylation pattern and spindle integrity in murine oocytes. PLoS One 11:e0162722
  Demond H, Trapphoff T, Dankert D, Heiligentag M, Grümmer R, Horsthemke B, Eichenlaub-Ritter U
  (Siehe online unter https://doi.org/10.1371/journal.pone.0162722)