In vitro maturation (IVM) - clinical follow up of pregnancies achieved by in vitro maturation and consecutive IVF and analysis of IVM oocytes in the human and a mouse model

• Antragsteller: Professor Dr. Thomas Haaf; Professor Dr. Thomas Strowitzki
• Fachliche Zuordnung: Reproduktionsmedizin, Urologie
• Förderung: Förderung von 2008 bis 2014
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 58733678

• Erstellungsjahr: 2017

Zusammenfassung der Projektergebnisse

In vitro maturation (IVM) of oocytes in IVF cycles (in vitro fertilization) is a new but promising technique. It may offer low risk IVF procedures especially in patients who are at high risk of ovarian hyperstimulation syndrome (OHSS). We have analysed the safety of IVM under several aspects: Pre- and perinatal data compared to a matched group of standard IVF-/ICSI pregnancies. - Two years follow up of IVM children and neurological testing according to Bayley score also compared to a matched group of standard IVF-/ICSI children. - Epimutation screening in individual human (IVM versus control) oocytes as well as in placental tissue and umbilical cord blood from children after IVM, IVF or ICSI. Up to now we couldn’t detect any negative effects of in vitro maturation of human oocytes. This data further confirm the safety of clinical use of this technique off assisted reproduction.

Projektbezogene Publikationen (Auswahl)

• (2008) In vitro maturation--a new therapeutic option in polycystic ovary syndrome. Gynakol Geburtshilfliche Rundsch 48:24-30
  von Wolff M, Eberhardt I, Strowitzki T
  
• (2011) Die In-vitro-Maturation in Deutschland. Gyn Endokrinol 9:229-232
  Strowitzki T, Rösner S
  
• (2012) In vitro maturation: a five-year experience. Acta Obstet Gynecol Scand 91:22-27
  Roesner S, von Wolff M, Eberhardt I, Beuter-Winkler P, Toth B, Strowitzki T
  
• (2013) Epigenetic disturbances in in vitro cultured gametes and embryos: implications for human assisted reproduction. Fertil Steril 99:632-641
  El Hajj N, Haaf T
  
• (2013) In vitro Maturation (IVM) of human oocytes. Arch Gynecol Obstet 288:971-97
  Strowitzki T
  
• (2013) In-vitro-Maturation (IVM). In Diedrich K, Ludwig M, Griesinger G (Hrsg.) Reproduktionsmedizin, Springer Verlag Berlin Heidelberg, ISBN 978-3-642-30180-3, pp 247-253
  Strowitzki T, Diedrich K
  
• (2014) Human in vitro oocyte maturation is not associated with increased imprinting error rates at LIT1, SNRPN, PEG3 and GTL2. Hum Reprod 29:1995-2005
  Kuhtz J, Romero S, De Vos M, Smitz J, Haaf T, Anckaert E
  
• (2015) In vitro maturation of oocytes is not associated with altered deoxyribonucleic acid methylation patterns in children from in vitro fertilization or intracytoplasmic sperm injection. Fertil Steril 103:720-727
  Pliushch G, Schneider E, Schneider T, El Hajj N, Rösner S, Strowitzki T, Haaf T
  
• (2017) Time-lapse imaging reveals differences in growth dynamics of embryos after in vitro maturation compared with conventional stimulation. Fertil Steril 107:606-612
  Roesner S, Dietrich JE, Weigert J, Montag M, Toth B, Strowitzki T
  
• (2017) Two-year development of children conceived by IVM: a prospective controlled single-blinded study. Hum Reprod 32:1341-1350
  Roesner S, von Wolff M, Elsaesser M, Roesner K, Reuner G, Pietz J, Bruckner T, Strowitzki T