Rhizobacterial volatiles: structure elucidation and impact on gene expression in Arabidopsis thaliana

• Antragstellerinnen / Antragsteller: Professor Dr. Wittko Francke (†); Professorin Dr. Birgit Piechulla
• Fachliche Zuordnung: Biochemie und Biophysik der Pflanzen
• Förderung: Förderung von 2008 bis 2012
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 73053051

• Erstellungsjahr: 2012

Zusammenfassung der Projektergebnisse

More than previously expected, bacteria emit a wealth of volatiles. A literature survey revealed ca. 800 microbial volatiles are known to date. Also rhizobacteria have the capability of volatile emission indicating an importance in belowground inter-/intraorganismal communication. The blend of Serratia odorifera 4Rx13, isolated from the rhizosphere of Brassica napus, comprised ca. 100 compounds. So far, the biological significance of 5 volatiles was determined, however, the relevance of many more compounds needs to be investigated in the future. The major compound of S. odorifera 4Rx13 was elucidated. It is a polymethylated bicyclic octadiene hydrocarbon (C16H26) with an unusual and unique structure. Its biosynthetic pathway is completely unknown as well as its biological significance. The morphological, physiological and molecular reactions of A. thaliana upon bacterial blend exposure were explored and for the first time a volatile dependent activation of a WRKY transduction chain was demonstrated. mVAMP induced transcription is a novel activation mode demanding for substantial additional investigations along this line, including signaling pathway cross talks and networks.

Projektbezogene Publikationen (Auswahl)

• Bacterial volatiles and their action potential Applied Microbiology and Biotechnology 81: 1001- 1012, 2008
  Kai M., Haustein M., Molina Martir F., Petri A., Scholz B., Piechulla B.
  
• Plant growth promotion due to rhizobacterial volatiles – An effect of CO2? FEBS Letters 583: 3473-3477, 2009
  Kai M., Piechulla B.
  
• Belowground volatiles facilitate interactions between plant roots and soil organisms Planta 231: 499-506, 2010
  Wenke K., Kai M., Piechulla B.
  
• Octamethylbicyclo(3.2.1)octadienes from Serratia odorifera Angewandte Chemie 122: 2053-2054, 2010; Angewandte Chemie Int. Ed. 49: 2009-2010, 2010
  Von Reuss S., Kai M., Piechulla B., Francke W.
  
• Serratia odorifera: Analysis of volatile emission and biological impact of volatile compounds on Arabidopisis thaliana Applied Microbiology and Biotechnology 88: 965-976, 2010
  Kai M., Crespo E., Cristescu S.M., Harren F.J.M., Francke W., Piechulla B.
  
• Bacterial volatiles mediating information between bacteria and plants In Bio-Communication in Plants, eds G. Witzany, F. Baluska, Springer, Berlin Heidelberg, pp. 327-347, 2012
  Wenke K., Weise T., Kai M., Warnke R., Valverde C., Wanke D., Kai M., Piechulla B.
  
• Volatile mediated interactions between bacteria and fungi in the soil Journal of Chemical Ecology 38: 665-703, 2012
  Effmert U., Kalderas J., Warnke R., Piechulla B.
  
• Volatile organic compounds of Xanthomonas campestris pv. vesicatoria as fungistatic agents Beilstein Journal of Organic Chemistry 8: 579-596 2012
  Weise T, Kai, M., Gummerson A.,von Reuß S., Sklorz M., Zimmermann R., Francke W., Piechulla B.
  
• Volatile-associated molecular pattens of two growth-inhibiting rhizobacteria commonly engage AtWRKY18 function. The Plant Journal 70: 445-459, 2012
  Wenke K., Wanke D, Kilian J., Berendzen K., Harter K., Piechulla B