Defining the role of the two vacuolar proton-pumps during stress responses
Final Report Abstract
The productivity of higher plants is linked to their ability to buffer changes in the concentrations of essential and toxic ions by vacuolar sequestration. Although it is generally assumed that transport across the tonoplast is energized by two proton pumps, the vacuolar H+-ATPase (V-ATPase) and the vacuolar H+-pyrophosphatase (V-PPase), their functional relation and relative contributions to vacuolar and cytosolic pH-homeostasis during cold- and osmotic stress had remained largely unaddressed. Funding of our project within FOR1061 has allowed us to make important contributions concerning the energetization of vacuolar transport and vacuolar biogenesis. Perhaps even more important, we have established very fruitful collaborations that will last much longer then the funding period.
Publications
- (2013). PDMP induces rapid changes in vacuole morphology in Arabidopsis root cells. J. Exp. Bot. 64: 529–540
Krüger, F., Krebs, M., Viotti, C., Langhans, M., Schumacher, K., and Robinson, D.G.
(See online at https://doi.org/10.1093/jxb/ers345) - (2013). The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis. Plant Cell 25: 3434–3449
Viotti, C., Krüger, F., Krebs, M., Neubert, C., Fink, F., Lupanga, U., Scheuring, D., Boutte, Y., Frescatada-Rosa, M., Wolfenstetter, S., Sauer, N., Hillmer, S., Grebe, M., and Schumacher, K.
(See online at https://doi.org/10.1105/tpc.113.114827) - (2014). Control of vacuolar dynamics and regulation of stomatal aperture by tonoplast potassium uptake. Proc. Natl. Acad. Sci. USA 111: E1806–14
Andrés, Z., Pérez-Hormaeche, J., Leidi, E.O., Schlücking, K., Steinhorst, L., McLachlan, D.H., Schumacher, K., Hetherington, A.M., Kudla, J., Cubero, B., and Pardo, J.M.
(See online at https://doi.org/10.1073/pnas.1320421111) - (2014). pH in the plant endomembrane system - an import and export business. Curr. Opin. Plant Biol. 22: 71–76
Schumacher, K.
(See online at https://doi.org/10.1016/j.pbi.2014.09.005) - (2015). Job Sharing in the Endomembrane System: Vacuolar Acidification Requires the Combined Activity of V-ATPase and V-PPase. Plant Cell 27: 3383–3396
Kriegel, A., Andrés, Z., Medzihradszky, A., Krüger, F., Scholl, S., Delang, S., Patir-Nebioglu, M.G., Gute, G., Yng, H., Murphy, A.S., Peer, W.A., Pfeiffer, A., Krebs, M., Lohmann, J.U., and Schumacher, K.
(See online at https://doi.org/10.1105/tpc.15.00733)
