Conformational dynamics of RNA regulation elements studied by NMR spectroscopy (A01)

• Subject Area: Biophysics
• Term: from 2011 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 161793742

Project A1 focuses on NMR-spectroscopic characterization of riboswitch RNAs. We aim to understand riboswitches in increasingly complex environment, starting from isolated aptamer domains in vitro towards the translation initiation machinery in vivo. To gain high-resolution structural information of these macromolecular complexes, we develop biochemical methods such as site-specific modification of single nucleotides, and NMR-spectroscopic methods, such as hetero-nuclei direct-detected experiments. Exemplified by further regulative RNAs we demonstrate the broad applicability of our methods in the field of RNA-based regulation.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 902: Molecular Principles of RNA-based Regulation
• Applicant Institution: Goethe-Universität Frankfurt am Main
• Project Head: Professor Dr. Harald Schwalbe