TIRF Mikroskopiesystem

• Subject Area: Basic Research in Biology and Medicine
• Term: Funded in 2012
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 223638783

• Final Report Year: 2016

Final Report Abstract

The TIRF/FRAP system has been essential for the successful development of the following projects: Demonstration of the impact of GAR22beta on the regulation of focal adhesion dynamics and directional cell motility. Demonstration of the essential role of the LSP1-mysoin1e bimolecular complex in the regulation of Fcgamma receptor-mediated phagocytosis. Characterization of the impact of surface topography on the differentiation and motility of mesenchymal stem cells. Characterization of the role of SigR1 receptor in the development of autophagy and lipid raft disturbancies in ALS pathology.

Publications

• 2014). Loss of function of the ALS protein SigR1 leads to ER pathology associated with defective autophagy and lipid raft disturbances. Cell Death Dis 5, e1290
  Vollrath, J.T., Sechi, A., Dreser, A., Katona, I., Wiemuth, D., Vervoorts, J., Dohmen, M., Chandrasekar, A., Prause, J., Brauers, E., Jesse, C.M., Weis, J., and Goswami, A.
  (See online at https://doi.org/10.1038/cddis.2014.243)
• (2015). Crucial role for the LSP1- myosin1e bimolecular complex in the regulation of Fcgamma receptor-driven phagocytosis. Mol Biol Cell 26, 1652-1664
  Maxeiner, S., Shi, N., Schalla, C., Aydin, G., Hoss, M., Vogel, S., Zenke, M., and Sechi, A.S.
  (See online at https://doi.org/10.1091/mbc.e14-05-1005)
• (2015). Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages. Biomaterials 61, 316-326
  Abagnale, G., Steger, M., Nguyen, V.H., Hersch, N., Sechi, A., Joussen, S., Denecke, B., Merkel, R., Hoffmann, B., Dreser, A., Schnakenberg, U., Gillner, A., and Wagner, W.
  (See online at https://doi.org/10.1016/j.biomaterials.2015.05.030)
• (2016). GAR22beta regulates cell migration, sperm motility, and axoneme structure. Mol Biol Cell 27, 277-294
  Gamper, I., Fleck, D., Barlin, M., Spehr, M., Sayad, S.E., Kleine, H., Maxeiner, S., Schalla, C., Aydin, G., Hoss, M., Litchfield, D.W., Luscher, B., Zenke, M., and Sechi, A.
  (See online at https://doi.org/10.1091/mbc.e15-06-0426)