Dendritogenesis of neocortical pyramidal cells and interneurons: regulation by AMPA and NMDA receptors.
Zusammenfassung der Projektergebnisse
Taken together, the major aims of the proposal were reached: we put the TARPs on the scene in a developmental context. Only the trafficking-competent TARPs were effective. This indicates that the increase of AMPARs at the cell surface and the subsequent increase of the amplitude of AMPAR-mediated currents (measured as calcium events) is the critical trigger for growth. We found evidence for a role of the endogenous AMPA receptors in promoting selectively the growth of apical dendrites of pyramidal neurons, in part during specific time windows and for neurons in specific cortical layers. We proved a role for endogenous γ-8, and presented evidence that the C-terminus is important. Interestingly, none of the TARPs modified the dendritic growth of the multipolar inhibitory interneurons. Although we have no evidence yet for a role of TARPs in interneurons, the positive aspect is that these neurons served as a perfect internal control.
Projektbezogene Publikationen (Auswahl)
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(2014) Auxiliary subunits: shepherding AMPA receptors to the plasma membrane. Membranes (Basel) 4:469-490
Haering SC, Tapken D, Pahl S, Hollmann M
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(2014) Regulation of postnatal neocortical pyramidal cell dendritic architecture by type I TARPs. 9th FENS Meeting, Milano
Hamad MIK, Jack A, Klatt O, Hollmann M, Wahle P
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(2014) The role of type-II TARPs for postnatal development of dendritic architecture of neocortical neurons. 9th FENS Meeting, Milano
Jack A, Hamad MIK, Klatt O, Hollmann M, Wahle P
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(2014) Type I TARPs promote dendritic growth of early postnatal neocortical pyramidal cells in organotypic cultures. Development 141:1737-1748
Hamad MI, Jack A, Klatt O, Lorkowski M, Strasdeit T, Kott S, Sager C, Hollmann M, Wahle P
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(2015) Improving AM ester calcium dye loading efficiency. J Neurosci Methods 240:48-60
Hamad MI, Krause M, Wahle P
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(2016) Ionotropic glutamate receptors: Which ones, when, and where in the mammalian neocortex. J Comp Neurol. August 25, pages 1-58 [Epub ahead of print]
Hadzic M, Jack A, Wahle P
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(2016) Kainate receptor GluK2 elicits network activity and promotes dendritic growth in early postnatal rat visual cortex. 10th FENS Meeting, Copenhagen
Jack A, Syska C, Hamad MIK, Hollmann M Wahle P
