Plasma-Zell Interaktionen in der Dermatologie (Markierungsfreie IR-Bildgebung von Plasma-Zell Interaktionen in der Dermatolog)
Zusammenfassung der Projektergebnisse
Within the project “Plasma Cell Interactions in Dermatology” we examined the interaction of non-thermal atmospheric pressure plasma with skin and its components. Here, the identification and characterisation of the chemical effect of active plasma species onto basic biological material of the skin, whole cells, and artificial skin was aimed. The performed vibrational microspectroscopic experiments revealed several chemical alterations in the samples. Upon plasma treatment, formations of organic nitrates in components of the skin and in bacteria could be identified as well as plasma-mediated modifications of DNA and nucleobases. A pronounced plasma-induced oxidative effect on lipids and sulfur containing molecules such as amino acids or proteins was not only identified in biological material, but also in bacterial and eukaryotic cells as well as in artificial skin. Analysis of reaction kinetics of the identified chemical modifications allowed a time-dependent insight into the plasma-mediated impact. It was able to analyse the individual impact of plasma constituents for selected chemical alterations, gaining a closer insight into plasma-skin interactions. The obtained results can provide a basis for defining and optimizing parameters for a plasma treatment of skin in biomedical applications.
Projektbezogene Publikationen (Auswahl)
- Medical Applications of Vibrational Spectroscopy: Disease Characterization, Therapy Surveillance and Development of High-Resolution Technology, Ph.D. thesis, Ruhr-Universität Bochum (2013)
M. Mischo
- Synergistic effects of atmospheric pressure plasma-emitted components on DNA oligomers: a Raman spectroscopic study, J. Biophotonics 8, 918 (2015)
E. Edengeiser, J.-W. Lackmann, E. Bründermann, S. Schneider, J. Benedikt, J.E. Bandow, and M. Havenith
(Siehe online unter https://doi.org/10.1002/jbio.201400123) - Unraveling the interactions between cold atmospheric plasma and skin-components with vibrational microspectroscopy, Biointerphases 10, 029516 (2015)
K. Kartaschew, M. Mischo, S. Baldus, E. Bründermann, P. Awakowicz, and M. Havenith
(Siehe online unter https://doi.org/10.1116/1.4919610) - FTIR spectroscopy of cysteine as a ready-to-use method for the investigation of plasma-induced chemical modifications of macromolecules, J. Phys. D. Appl. Phys. 49, 084004 (2016)
F. Kogelheide, K. Kartaschew, M. Strack, S. Baldus, N. Metzler-Nolte, M. Havenith, P. Awakowicz, K. Stapelmann, and J.-W. Lackmann
(Siehe online unter https://doi.org/10.1088/0022-3727/49/8/084004)
