Hochgeschwindigkeits-Zellsortiersystem
Zusammenfassung der Projektergebnisse
FACS sorting was used to obtain discrete B cell subpopulations ranging in maturity from naive B cells to memory B cells in a study of DNA methylation programming during B cell maturation. These subpopulations were then used to observe a broad epigenetic programming of selective transcription factor binding sites coincident with the degree of B cell maturation. Comparisons were made between normal B cells and malignant B cells from 268 patients with chronic lymphocytic leukemia (CLL). Postoperative ileus (POI), the most frequent complication after intestinal surgery, depends on dendritic cells (DCs) and macrophages. FACS and cell sorting was used for their quantification and selection in a study that showed that CD103+CD11b+ DCs and the intestinal microbiome are a prerequisite for the activation of intestinal monocytes and macrophages and for dysregulating intestinal motility in POI. FACS was used to quantify the number of liver resident macrophages in a study to investigate the role of bile acids and their receptor farnesoid X on macrophage proliferation and response to viral infection in hepatocytes.
Projektbezogene Publikationen (Auswahl)
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„Complexity of the human memory B-cell compartment is determined by the versatility of clonal diversification in germinal centers.“ Proc Natl Acad Sci USA. 2015. 112(38)
Budeus B, Schweigle de Reynoso S, Przekopowitz M, Hoffmann D, Seifert M, Küppers R
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„Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.“ Proc Natl Acad Sci USA. 2015 112(6)
Seifert M, Przekopowitz M, Taudien S, Lollies A, Ronge V, Drees B, Lindemann M, Hillen U, Engler H, Singer BB, Küppers R
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„Recurrent alterations of TNFAIP3 (A20) in T-cell large granular lymphocytic leukemia.” Int J Cancer. 2015
Johansson P, Bergmann A, Rahmann S, Wohlers I, Scholtysik R, Przekopowitz M, Seifert M, Tschurtschenthaler G, Webersinke G, Jäger U, Siebert R, Klein-Hitpass L, Dührsen U, Dürig J, Küppers R
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„DNA methylation dynamics during B cell maturation underlie a continuum of disease phenotypes in chronic lymphocytic leukemia.“ Nat Genet. 2016
Oakes CC, Seifert M, Assenov Y, Gu L, Przekopowitz M, Ruppert AS, Wang Q, Imbusch CD, Serva A, Koser SD, Brocks D, Lipka DB, Bogatyrova O, Weichenhan D, Brors B, Rassenti L, Kipps TJ, Mertens D, Zapatka M, Lichter P, Döhner H, Küppers R, Zenz T, Stilgenbauer S, Byrd JC, Plass C
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„DNA methylation dynamics during B cell maturation underlie a continuum of disease phenotypes in chronic lymphocytic leukemia.“ Nat Genet. 2016
Oakes CC, Seifert M, Assenov Y, Gu L, Przekopowitz M, Ruppert AS, Wang Q, Imbusch CD, Serva A, Koser SD, Brocks D, Lipka DB, Bogatyrova O, Weichenhan D, Brors B, Rassenti L, Kipps TJ, Mertens D, Zapatka M, Lichter P, Döhner H, Küppers R, Zenz T, Stilgenbauer S, Byrd JC, Plass C
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„Immunoactivation induced by chronic viral infection inhibits viral replication and drives immunosuppression through sustained IFN-I responses.“ Eur J Immunol. 2016 46(2)
Honke N, Shaabani N, Merches K, Gassa A, Kraft A, Ehrhardt K, Häussinger D, Löhning M, Dittmer U, Hengel H, Recher M, Lang PA, Lang KS
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„Farnesoid X Receptor in Mice Prevents Severe Liver Immunopathology During Lymphocytic Choriomeningitis Virus Infection.“ Cell Physiol Biochem. 2017;41(1)
Honke N, Shaabani N, Hardt C, Krings C, Häussinger D, Lang PA, Lang KS, Keitel V
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„Irf4-dependent CD103+CD11b+ dendritic cells and the intestinal microbiome regulate monocyte and macrophage activation and intestinal peristalsis in postoperative ileus.“ Gut. 2017
Pohl JM, Gutweiler S, Thiebes S, Volke JK, Klein-Hitpass L, Zwanziger D, Gunzer M, Jung S, Agace WW, Engel DR, Kurts C
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„Natural killer T cells contribute to the control of acute retroviral infection.“ Retrovirology. 2017 14(1):5
Littwitz-Salomon E, Schimmer S, Dittmer U
