Ein label-freies optisches Sensorsystem in Silizium: Bestimmung der thermodynamischen und kinetischen Kenngrößen von Protein-Ligand Wechselwirkungen
Organische Molekülchemie - Synthese, Charakterisierung
Zusammenfassung der Projektergebnisse
The main objective of this project was to develop a biochemical sensing system based on silicon photonics and the corresponding biochemical assay techniques for the detection of selected proteins. The work was divided into three subprojects: • Optical biosensor system • Surface chemistry • Assay techniques One project partner mainly worked on APTES-silanized substrates and ATR crystals, which were modified with crosslinkers and peptides. Protocols developed in this activity were used for the SOI-based sensor systems surface functionalization. The development of the label-free bio sensor system comprises of the sensor itself, a TDM- like control for fast read-out and fixed-wavelength operation. The device is based on evanescent field sensing and ring resonators as sensing elements. A heating electrode is integrated to tune the device by using the thermo-optical effect of silicon. To enable multiparameter analysis, several ring resonators were coupled to a single bus waveguide. By applying a temporal modulation signal in a fix switching scheme to each ring it was demonstrated that a serial array of ring elements could be read-out simultaneously at a speed of 100 measurements / s for each ring. This measurement speed makes the kinetics of proteinligand interaction accessible. Furthermore, the fixed wavelength operation enables the realization of a cost effective sensor device with large potential for a handheld appliance. In focus of the assay technique development we figure out a list of peptides all recognizing an anti-GST IgG antibody but with different affinities spanning from middle/high nanomolar up to micromolar affinity. Furthermore, to pave the way for a practical usage of the label-free bio sensor system we engineer a microfluidic surface functionalization and sensing technique successfully.
Projektbezogene Publikationen (Auswahl)
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Multiplexed single wavelength bio sensor for low cost applications, in Advanced Photonics 2015, OSA Technical Digest (online) (Optical Society of America, 2015), paper SeT1C.4.
Jäger, Matthias; Volkmann, Danny; Bruns, Jürgen & Petermann, Klaus
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Antifouling coatings on SOI microring resonators for bio sensing applications, Sensors Actuators, B Chem., vol. 223, pp. 400–405, 2016
Jäger, Matthias; Becherer, Tobias; Bruns, Jürgen; Haag, Rainer & Petermann, Klaus
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IR-spektroskopische Charakterisierung von organisch-funktionalisierten Monolagen und Immobilisierung von Peptiden auf Silizium Oberflächen, doctoral thesis, TU Berlin, November 2018
Anne Borowski
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TDM-controlled ring resonator arrays for fast, fixed-wavelength optical biosensing, Optics Express, vol. 26, no. 17, pp. 22356–22365, 2018
Moock, P.; Kasper, L.; Jäger, M.; Stolarek, D.; Richter, H.; Bruns, J. & Petermann, K.
