SPP 1934:  Dispersitäts-, Struktur- und Phasenänderungen von Proteinen und biologischen Agglomeraten in biotechnologischen Prozessen

• Subject Area: Thermal Engineering/Process Engineering ; Agriculture, Forestry and Veterinary Medicine ; Biology ; Chemistry ; Computer Science, Systems and Electrical Engineering ; Materials Science and Engineering ; Medicine
• Term: from 2016 to 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 273937032

In the frame of the priority program SPP 1934 “Dispersity, structure and phase changes of proteins and bio agglomerates in biotechnological processes” the stressing of sensitive proteins and bio agglomerates by the process environment during fermentation, down-stream processing and formulation is investigated. The priority program is addressed to scientists working in the fields of chemical and process engineering, especially particle technology and biochemical engineering, as well as of biology. These research fields are connected today not strongly enough. Therefore, in the priority program these research fields are cross-linked intensively. The SPP coordinator has still to fulfil several tasks: For the strong support of the networking regular meetings between the involved working groups (principal investigators and co-workers/PhD-students) have to be organized. In this priority program especially also workshops without principal investigators shall take place. Moreover the organization of meetings regarding different interdisciplinary themes as well as of summer schools and the invitation of guest scientists belong to the coordinator tasks. Besides the organisation of the meetings the accounting of the meetings and of the stays of the guest scientists as well as the documentation of the results of the priority program in the internet are part of the coordinators duty. For the accomplishment of the time consuming tasks and for travelling to the meetings financial and personal resources are required.

• DFG Programme: Priority Programmes
• International Connection: Netherlands

Projects

• Amyloid protein aggregates of beta-lactoglobulin and their behavior along the process chain (Applicants Keppler, Julia ;  Schwarz, Karin ;  Steffen-Heins, Anja )
• Behavior of proteins inside microfluidic channels (Applicant Hirsch, Sören )
• Breakage of unstable protein crystals in mechanical solid-liquid separation processes (Applicant Nirschl, Hermann )
• Central project - Cross-project research, production and characterization of new model proteins (Applicants Jahn, Dieter ;  Krull, Rainer ;  Kwade, Arno )
• Coordination of the DFG Priority Programme DiSPBiotech (Applicant Kwade, Arno )
• Coordination of the DFG Priority Programme DiSPBiotech (Applicant Kwade, Arno )
• Correlation of structure and properties of protein crystals in consideration of downstream processing and formulation (Applicants Biedendieck, Rebekka ;  Schallmey, Anett ;  Schilde, Carsten )
• During a protein spray drying process, thermal and mechanical stresses are exerted on the proteins leading to degradation. We hypothesize that in situ measurements of the droplet size, temperature distribution inside droplets and UV fluorescence will provide degradation kinetics. (Applicants Mädler, Lutz ;  Wriedt, Thomas )
• EnzymAgglo - Multiscale model-based investigation of functional enzyme and protein agglomerates for biotechnological applications. Part 2: From structure to function (Applicants Heinrich, Stefan ;  Zeng, An-Ping )
• Experimental and numerical investigations on the effects of cavitation and flow-related collisions on aggregate formation and loss of activity after cell disruption in the high-pressure homogeniser using the model systems lipase or beta-lactoglobulin as examples (Applicant Karbstein, Heike )
• Generalized morphological modeling of aggregating, filamentous microorganisms (Applicant Briesen, Heiko )
• Green Bioprinting - Novel Environment for Agglomeration of Plant Cells (Applicants Lode, Anja ;  Steingroewer, Juliane )
• Impact and control of mechanical stress on protein structures during formulation in premix-emulsification (Applicants Drusch, Stephan ;  Fritsching, Udo ;  Wagemans, Anja Maria )
• In situ Studies of Structure Changes of Biomolecules at Interfaces and Under Fluidmechanical Stress (Applicant Peukert, Wolfgang )
• Influence of thermomechanical stresses on structural and functional changes of highly concentrated protein systems in extrusion processing (Applicant Karbstein, Heike )
• Innovative Pharmaceutical Stabilization and Formulation Techniques for Protein Drugs and Their Influence on Sequence and Structure (Applicants Imhof, Ph.D., Diana ;  Kühl, Toni ;  Lamprecht, Alf ;  Wagner, Karl G. )
• Interaction of mechanical stresses and productivity of biological agglomerates in stirred fermenters (Applicant Kraume, Matthias )
• Investigation and modeling of the mechanical and surface-induced stress on pellets of filamentous microorganisms on Lechevalieria aerocolonigenes (Applicants Krull, Rainer ;  Kwade, Arno )
• Investigation of mechanisms in the formation of structures and of processing.structure-relationships in multiphase, highly concentrated protein systems (Applicant Kulozik, Ulrich )
• Modeling-based control of the crystallizability of proteins in biotechnological processes (Applicants Hubbuch, Jürgen ;  Weuster-Botz, Dirk )
• Nucleation Kinetics during Suspension Crystallization of Proteins (Applicant Kind, Matthias )
• Protein adsorption at gas-liquid interfaces: Mechanism and applications (Applicant Schembecker, Gerhard )
• Structure, conformation and phase changes of virus-like particles (VLPs) under chemical and mechanical stress in presence of nucleic acids (Applicants Hubbuch, Jürgen ;  Wenzel, Wolfgang )
• The effect of shear forces on the morphogenetic gene network, cell integrity, microscopic and macroscopic morphology of Aspergillus niger and on the formation rate of intra- and extracellular products (Applicants King, Rudibert ;  Meyer, Vera )

• Spokesperson: Professor Dr.-Ing. Arno Kwade