Project Details
Projekt
Tumorigenic cytokine networks during colon carcinogenesis depend on sphingosine-1-phosphate receptor signalling
Subject Area
Public Health, Healthcare Research, Social and Occupational Medicine
Term
from 2016 to 2021
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 280163318
The immune contexture in colorectal cancer emerges as an important prognostic factor for patient survival. Accordingly, the relative presence of adaptive immune cell subpopulations in intestinal tumors marks either good prognosis (cytotoxic CD8+ T cells, TH1-polarized CD4+ T cells) or bad prognosis (TH17-polarized CD4+ T cells). Lymphocyte plasticity in colorectal carcinogenesis depends on master inflammatory regulators in myeloid cells such as NF-kappaB, STAT3 or STAT1, and their downstream mediators, including the cytokines IL-1beta, IL-6, IL-23, and TNF-alpha, which promote, and IL-12 or type I interferons, which restrict carcinogenesis. The production of these cytokines is initially triggered by exogenous or endogenous danger signals. Signaling through pattern recognition receptors recognizing such danger signals receives substantial regulatory input through other signaling pathways. We follow the hypothesis that the sphingolipid sphingosine-1-phosphate (S1P) critically regulates inflammatory cytokines in colorectal cancer by signaling through specific G protein-coupled receptors (S1PRs). Enhanced S1P levels were previously connected to colon carcinogenesis. Own data suggest that particularly S1PR1 and S1PR4 are involved in tumor-associated inflammation. S1PR1 expression in tumor-associated macrophages in mammary carcinoma and fibrosarcoma promoted lymphangiogenesis and metastasis through IL-1beta secretion. S1PR4 promoted TH17 development in breast tumors and limited type I interferon production. Based on this evidence, we propose a tumor-promoting role for S1PR4 and a dominating role of S1PR1 signaling in colorectal cancer metastasis. Specifically, we investigate if S1PR1 expression by macrophages promotes lymph node metastasis via IL-1beta in a model of metastatic colon cancer. Additionally we ask whether S1PR4-/- mice show a lower incidence of colitis-associated cancer and analyze the underlying altered cytokine networks and myeloid cell/lymphocyte plasticity. Our studies will identify new regulators of tumor-associated inflammation and may suggest potential pharmacological targets to interfere with inflammatory events that promote colon carcinogenesis and metastasis.
DFG Programme
Research Units
Subproject of
FOR 2438:
Cell Plasticity in Colorectal Carcinogenesis
