Molekulare und funktionale Untersuchung des Mechanismus, der die zytoplasmatische Strömung in Arabidopsis thaliana antreibt
Zusammenfassung der Projektergebnisse
In this work, we aimed to understand the role of XI myosins in driving cytoplasmic streaming and its function in plants. As redundancies make genetic approaches very challenging, we took a complementary approach and mobilized specific organelles or RNPs in the myosin xi-1 xi-2 xi-k triple mutant (3KO) by expressing chimeric XI-K myosins in which the cargo binding domains were replaced by proteins or fragments residing in the organelle/RNP of interest. The corresponding XIKΔGTD-anchor proteins rescued the actin phenotype and the motility of the respective organelles. Mobilizing mitochondria, peroxisomes, late endosomes, secretory vesicles, and Golgi rescued the morphological leaf size and trichome phenotypes. Attempts to analyze the co-mobilization of other organelles in stable transgenic plants failed in most cases due to silencing effects. Studying the co-mobilization by transiently expressing markers in stable XIKΔGTD-anchor lines rescuing the 3KO phenotype suggest a differential co-mobilization pattern. We also aimed to distinguish between the mobilization of organelles and the streaming of the liquid phase of the cytoplasm using FRAP or lumazine synthase, however, did not succeed.
Projektbezogene Publikationen (Auswahl)
- (2021). Unravelling the molecular basis of the dominant negative effect of myosin XI tails on P-bodies. PloS one, 16(5), e0252327
Stephan, L., Jakoby, M., Das, A., Koebke, E., & Hülskamp, M.
(Siehe online unter https://doi.org/10.1371/journal.pone.0252327)
