EXC 294:  Centre for Biological Signalling Studies (bioss) - From Analysis to Synthesis

• Subject Area: Basic Research in Biology and Medicine
• Term: from 2007 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 39236281

• Final Report Year: 2019

Final Report Abstract

We are living in the age of information, and information or signal processing are also of fundamental importance for the development and maintenance of cellular live on this planet. The Centre for Biological Signalling Studies (BIOSS) in Freiburg was founded to reach a more comprehensive understanding of the composition and function of biological signalling systems by addressing the seven basic questions of signalling science: “Who is involved and interacts with whom and what is the result of signalling? How does this signalling work and where does it take place? When does the specific signalling event occur and how much signals are necessary for a defined response?” The BIOSS scientists addressing these questions by applying a new research strategy “from analysis to synthesis” combining biological signalling studies with synthetic biology. For this, BIOSS set up a highly interdisciplinary research program bridging 5 faculties of the University of Freiburg and 2 non-university institutions. BIOSS funded 130 research projects over the last 11 years. In its second funding period BIOSS also established two new focus areas on nanoscale membrane signalling and oncogenic signalling. The productivity of the BIOSS signalling research community is indicated by the ca. 200 BIOSS publications each year. With the Signalhaus Freiburg, BIOSS has established a new research building at the University of Freiburg that is by now fully equipped and harbours most of the BIOSS infrastructure such as the BIOSS office, the BIOSS toolbox and the BIOSS signalling factory. Furthermore, the laboratories of the iGEM student teams and the teaching rooms for the “biology meets engineering” program are part of this building. BIOSS has successfully implemented most of its planned measures. BIOSS supported the work of 4 independent postdocs, 8 Junior groups and established 9 new professorships at the University of Freiburg. Importantly, as promised in the initial application BIOSS reached gender equality in these recruitments. Project funding and new personnel have established BIOSS as a highly visible research centre combining signalling science with synthetic approaches. Four of the new professors are working in the field of synthetic and chemical biology at the Faculty of Biology and two at the Faculty of chemistry and two more at the Faculty of Medicine. With the help of BIOSS, the University of Freiburg is now one of the nationally and internationally recognized centres for biological signalling research.

Publications

• Autophagy influences glomerular disease susceptibility and maintains podocyte homeostasis in aging mice. J Clin Invest 2010
  Hartleben B, Gödel M, Meyer-Schwesinger C, Liu S, Ulrich T, Köbler S, Wiech T, Grahammer F, Arnold SJ, Lindenmeyer MT, Cohen CD, Pavenstädt H, Kerjaschki D, Mizushima N, Shaw AS, Walz G, Huber TB
  (See online at https://doi.org/10.1172/JCI39492)
• Covering a broad dynamic range: Information Processing at the Eurythropoietin Receptor. Science 2010
  Becker V, Schilling M, Bachmann J, Baumann U, Raue A, Maiwald T, Timmer J, Klingmüller U
  (See online at https://doi.org/10.1126/science.1184913)
• Functional Modules and Structural Basis of Conformational Coupling in Mitochondrial Complex I. Science 2010
  Hunte C, Zickermann V, Brandt U
  (See online at https://doi.org/10.1126/science.1191046)
• Microscopy with self-reconstructing beams. Nat. Photonics 2010
  Fahrbach FO, Simon P, Rohrbach A
  (See online at https://doi.org/10.1038/nphoton.2010.204)
• Mitochondrial protein import: from proteomics to functional mechanisms. Nat Rev Mol Cell Biol 2010
  Schmidt O, Pfanner N, Meisinger C
  (See online at https://doi.org/10.1038/nrm2959)
• Native GABAB receptors are heteromultimers with a family of auxiliary subunits. Nature 2010
  Schwenk J, Metz M, Zolles G, Turecek R, Fritzius T, Bildl W, Tarusawa E, Kulik A, Unger A, Ivankova K, Seddik R, Tiao JY, Rajalu M, Trojanova J, Rohde V, Gassmann M, Schulte U, Fakler B, Bettler B
  (See online at https://doi.org/10.1038/nature08964)
• Oligomeric organisation of the B-cell antigen receptor on resting cells. Nature 2010
  Yang J, Reth M
  (See online at https://doi.org/10.1038/nature09357)
• Transcriptional control of gene expression by micro-RNAs. Cell. 2010
  Khraiwesh B, Arif MA, Seumel GI, Ossowski S, Weigel D, Reski R, Frank W
  (See online at https://doi.org/10.1016/j.cell.2009.12.023)
• Voltage control for B cell activation. Nat. Immunol 2010
  Dick T, Reth M
  (See online at https://doi.org/10.1038/ni0310-191)
• BCL6 enables Ph+ acute lymphoblastic leukaemia cells to survive BCR-ABL1 kinase inhibition. Nature. 2011
  Duy C, Hurtz C, Shojaee S, Cerchietti L, Geng H, Swaminathan S, Klemm L, Kweon SM, Nahar R, Braig M, Park E, Kim YM, Hofmann WK, Herzog S, Jumaa H, Koeffler HP, Yu JJ, Heisterkamp N, Graeber TG, Wu H, Ye BH, Melnick A, Müschen M
  (See online at https://doi.org/10.1038/nature09883)
• Emerging biomedical applications of synthetic biology. Nat Rev Genet. 2011
  Weber W, Fussenegger M
  (See online at https://doi.org/10.1038/nrg3094)
• Evidence for interstitial carbon in nitrogenase FeMo cofactor. Science. 2011
  Spatzal T, Aksoyoglu M, Zhang L, Andrade SL, Schleicher E, Weber S, Rees DC, Einsle O
  (See online at https://doi.org/10.1126/science.1214025)
• Heterochromatin boundaries are hotspots for de novo kinetochore formation. Nat Cell Biol 2011
  Olszak A, van Essen D, Pereira AJ, Diehl S, Manke T, Maiato H, Saccani S, Heun P
  (See online at https://doi.org/10.1038/ncb2272)
• mTORC1 activation in podocytes is a critical step in the development of diabetic nephropathy in mice. J Clin Invest. 2011
  Inoki K, Mori H, Wang J, Suzuki T, Hong S, Yoshida S, Blattner SM, Ikenoue T, Rüegg MA, Hall MN, Kwiatkowski DJ, Rastaldi MP, Huber TB, Kretzler M, Holzman LB, Wiggins RC, Guan KL
  (See online at https://doi.org/10.1172/jci44771)
• N2O binding at a [4Cu:2S] copper–sulphur cluster in nitrous oxide reductase. Nature 2011
  Pomowski A, Zumft WG, Kroneck PMH, Einsle O
  (See online at https://doi.org/10.1038/nature10332)
• pH-dependent gating in a FocA formate channel. Science. 2011
  Lü W, Du J, Wacker T, Gerbig-Smentek E, Andrade SL, Einsle O
  (See online at https://doi.org/10.1126/science.1199098)
• Photoconversion and Nuclear Trafficking Cycles Determine Phytochrome A's Response Profile to Far-Red Light. Cell. 2011
  Rausenberger J, Tscheuschler A, Nordmeier W, Wüst F, Timmer J, Schäfer E, Fleck C, Hiltbrunner A
  (See online at https://doi.org/10.1016/j.cell.2011.07.023)
• Regulation of mitochondrial protein import by cytosolic kinases. Cell. 2011
  Schmidt O, Harbauer AB, Rao S, Eyrich B, Zahedi RP, Stojanovski D, Schönfisch B, Guiard B, Sickmann A, Pfanner N, Meisinger C
  (See online at https://doi.org/10.1016/j.cell.2010.12.015)
• Role of mTOR in podocyte function and diabetic nephropathy in humans and mice. J Clin Invest. 2011
  Gödel M, Hartleben B, Herbach N, Liu S, Zschiedrich S, Lu S, Debreczeni-Mór A, Lindenmeyer MT, Rastaldi MP, Hartleben G, Wiech T, Fornoni A, Nelson RG, Kretzler M, Wanke R, Pavenstädt H, Kerjaschki D, Cohen CD, Hall MN, Rüegg MA, Inoki K, Walz G, Huber TB
  (See online at https://doi.org/10.1172/JCI44774)
• The Selaginella Genome Identifies Genetic Changes Associated with the Evolution of Vascular Plants. Science. 2011
  Banks JA, Nishiyama T, et al.
  (See online at https://doi.org/10.1126/science.1203810)
• A lineage of myeloid cells independent of Myb and hematopoietic stem cells. Science. 2012
  Schulz C, Gomez Perdiguero E, Chorro L, Szabo-Rogers H, Cagnard N, Kierdorf K, Prinz M, Wu B, Jacobsen SE, Pollard JW, Frampton J, Liu KJ, Geissmann F
  (See online at https://doi.org/10.1126/science.1219179)
• Algal genomes reveal evolutionary mosaicism and the fate of nucleomorphs. Nature. 2012
  Curtis BA, Tanifuji G, et al.
  (See online at https://doi.org/10.1038/nature11681)
• Chronic lymphocytic leukaemia is driven by antigen-independent cell-autonomous Signalling. Nature. 2012
  Dühren-von Minden M, Übelhart R, Schneider D, Wossning T, Bach MP, Buchner M, Hofmann D, Surova E, Follo M, Köhler F, Wardemann H, Zirlik K, Veelken H, Jumaa H
  (See online at https://doi.org/10.1038/nature11309)
• Cyanophora paradoxa genome elucidates origin of photosynthesis in algae and plants. Science. 2012
  Price DC, Chan CX, Yoon HS, Yang EC, Qiu H, Weber AP, Schwacke R, Gross J, Blouin NA, Lane C, Reyes-Prieto A, Durnford DG, Neilson JA, Lang BF, Burger G, Steiner JM, Löffelhardt W, Meuser JE, Posewitz MC, Ball S, Arias MC, Henrissat B, Coutinho PM, Rensing SA, Symeonidi A, Doddapaneni H, Green BR, Rajah VD, Boore J, Bhattacharya D
  (See online at https://doi.org/10.1126/science.1213561)
• Object-adapted optical trapping and shapetracking of energyswitching helical bacteria. Nat Photonics 2012
  Koch M, Rohrbach A
  (See online at https://doi.org/10.1038/nphoton.2012.232)
• Synthetic mammalian gene networks as a blueprint for the design of interactive biohybrid materials. Chem Soc Rev 2012
  Jakobus K, Wend S, Weber W
  (See online at https://doi.org/10.1039/c1cs15176b)
• Synthetic two-way communication between mammalian cells. Nat Biotechnol. 2012
  Bacchus W, Lang M, El-Baba MD, Weber W, Stelling J, Fussenegger M
  (See online at https://doi.org/10.1038/nbt.2351)
• Vertebrate kidney tubules elongate using a planar cell polarity-dependent, rosette-based mechanism of convergent extension. Nat Genet. 2012
  Lienkamp SS, Liu K, Karner CM, Carroll TJ, Ronneberger O, Wallingford JB, Walz G
  (See online at https://doi.org/10.1038/ng.2452)
• ViBE-Z: a framework for 3D virtual colocalization analysis in zebrafish larval brains. Nat Methods. 2012
  Ronneberger O, Liu K, Rath M, Ruess D, Mueller T, Skibbe H, Drayer B, Schmidt T, Filippi A, Nitschke R, Brox T, Burkhardt H, Driever W
  (See online at https://doi.org/10.1038/NMETH.2076)
• Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation. Cell. 2013
  Qiu J, Wenz LS, Zerbes RM, Oeljeklaus S, Bohnert M, Stroud DA, Wirth C, Ellenrieder L, Thornton N, Kutik S, Wiese S, Schulze-Specking A, Zufall N, Chacinska A, Guiard B, Hunte C, Warscheid B, van der Laan M, Pfanner N, Wiedemann N, Becker T
  (See online at https://doi.org/10.1016/j.cell.2013.06.033)
• Gene transfer from bacteria and archaea facilitated evolution of an extremophilic eukaryote. Science. 2013
  Schönknecht G, Chen WH, Ternes CM, Barbier GG, Shrestha RP, Stanke M, Bräutigam A, Baker BJ, Banfield JF, Garavito RM, Carr K, Wilkerson C, Rensing SA, Gagneul D, Dickenson NE, Oesterhelt C, Lercher MJ, Weber AP
  (See online at https://doi.org/10.1126/science.1231707)
• Inhibition of mTORC1 by astrin and stress granules prevents apoptosis in cancer cells. Cell. 2013
  Thedieck K, Holzwarth B, Prentzell MT, Boehlke C, Kläsener K, Ruf S, Sonntag AG, Maerz L, Grellscheid SN, Kremmer E, Nitschke R, Kuehn EW, Jonker JW, Groen AK, Reth M, Hall MN, Baumeister R
  (See online at https://doi.org/10.1016/j.cell.2013.07.031)
• Matching cellular dimensions with molecular sizes. Nat Immunol. 2013
  Reth M
  (See online at https://doi.org/10.1038/ni.2621)
• Pan genome of the phytoplankton Emiliania underpins its global distribution. Nature. 2013
  Read BA, Kegel J, et al.
  (See online at https://doi.org/10.1038/nature12221)
• Pou5f1 transcription factor controls zygotic gene activation in vertebrates. Science. 2013
  Leichsenring M, Maes J, Mössner R, Driever W, Onichtchouk D
  (See online at https://doi.org/10.1126/science.1242527)
• The nucleoplasmin homolog NLP mediates centromere clustering and anchoring to the nucleolus. Mol Cell. 2013
  Padeken J, Mendiburo MJ, Chlamydas S, Schwarz HJ, Kremmer E, Heun P
  (See online at https://doi.org/10.1016/j.molcel.2013.03.002)
• Amyloid-beta peptide induces mitochondrial dysfunction by inhibition of preprotein maturation. Cell Metab. 2014
  Mossmann D, Vögtle FN, Taskin AA, Teixeira PF, Ring J, Burkhart JM, Burger N, Pinho CM, Tadic J, Loreth D, Graff C, Metzger F, Sickmann A, Kretz O, Wiedemann N, Zahedi RP, Madeo F, Glaser E, Meisinger C
  (See online at https://doi.org/10.1016/j.cmet.2014.07.024)
• Cell cycle-dependent regulation of mitochondrial preprotein translocase. Science. 2014
  Harbauer AB, Opalinska M, Gerbeth C, Herman JS, Rao S, Schönfisch B, Guiard B, Schmidt O, Pfanner N, Meisinger C
  (See online at https://doi.org/10.1126/science.1261253)
• Differentiation of type 1 ILCs from a common progenitor to all helper-like innate lymphoid cell lineages. Cell. 2014
  Klose CS, Flach M, Möhle L, Rogell L, Hoyler T, Ebert K, Fabiunke C, Pfeifer D, Sexl V, Fonseca-Pereira D, Domingues RG, Veiga-Fernandes H, Arnold SJ, Busslinger M, Dunay IR, Tanriver Y, Diefenbach A
  (See online at https://doi.org/10.1016/j.cell.2014.03.030)
• Functional architecture of an optic flow-responsive area that drives horizontal eye movements in Zebrafish. Neuron 2014
  Kubo F, Hablitzel B, Maschio MD, Driever W, Baier H, Arrenberg AB
  (See online at https://doi.org/10.1016/j.neuron.2014.02.043)
• Inefficient SRP interaction with a nascent chain triggers a mRNA quality control pathway. Cell. 2014
  Karamyshev AL, Patrick AE, Karamysheva ZN, Griesemer DS, Hudson H, Tjon-Kon-Sang S, Nilsson I, Otto H, Liu Q, Rospert S, von Heijne G, Johnson AE, Thomas PJ
  (See online at https://doi.org/10.1016/j.cell.2013.12.017)
• Ligand binding to the FeMo-cofactor: structures of CO-bound and reactivated nitrogenase. Science. 2014
  Spatzal T, Perez KA, Einsle O, Howard JB, Rees DC
  (See online at https://doi.org/10.1126/science.1256679)
• Mechanism of Tc toxin action revealed in molecular detail. Nature. 2014
  Meusch D, Gatsogiannis C, Efremov RG, Lang AE, Hofnagel O, Vetter IR, Aktories K, Raunser S
  (See online at https://doi.org/10.1038/nature13015)
• Mgr2 functions as lateral gatekeeper for preprotein sorting in the mitochondrial inner membrane. Mol Cell. 2014
  Ieva R, Schrempp SG, Opalinski L, Wollweber F, Höß P, Heißwolf AK, Gebert M, Zhang Y, Guiard B, Rospert S, Becker T, Chacinska A, Pfanner N, van der Laan M
  (See online at https://doi.org/10.1016/j.molcel.2014.10.010)
• Microglia and brain macrophages in the molecular age: from origin to neuropsychiatric disease. Nat Rev Neurosci. 2014
  Prinz M, Priller J
  (See online at https://doi.org/10.1038/nrn3722)
• The protein import machinery of mitochondria-a regulatory hub in metabolism, stress, and disease. Cell Metab. 2014
  Harbauer AB, Zahedi RP, Sickmann A, Pfanner N, Meisinger C
  (See online at https://doi.org/10.1016/j.cmet.2014.01.010)
• A first step toward liposome-mediated intracellular bacteriophage therapy. Expert Opin Drug Deliv. 2015
  Nieth A, Verseux C, Barnert S, Süss R, Römer W
  (See online at https://doi.org/10.1517/17425247.2015.1043125)
• An optogenetic upgrade for the Tet-OFF system. Biotechnol Bioeng. 2015
  Müller K, Zurbriggen MD, Weber W
  (See online at https://doi.org/10.1002/bit.25562)
• antaRNA - Ant Colony Based RNA Sequence Design. Bioinformatics. 2015
  Kleinkauf R, Mann M, Backofen R
  (See online at https://doi.org/10.1093/bioinformatics/btv319)
• B cell antigen receptors of the IgM and IgD classes are clustered in different protein islands that are altered during B cell activation. Sci Signal. 2015
  Maity PC, Blount A, Jumaa H, Ronneberger O, Lillemeier BF, Reth M
  (See online at https://doi.org/10.1126/scisignal.2005887)
• B-Raf iInhibitors induce epithelial differentiation in BRAF-mutant colorectal cancer cells. Cancer Res. 2015
  Herr R, Köhler M, Andrlová H, Weinberg F, Möller Y, Halbach S, Lutz L, Mastroianni J, Klose M, Bittermann N, Kowar S, Zeiser R, Olayioye MA, Lassmann S, Busch H, Boerries M, Brummer T
  (See online at https://doi.org/10.1158/0008-5472.CAN-13-3686)
• Centrifugal microfluidic platforms: advanced unit operations and applications. Chem Soc Rev. 2015
  Strohmeier O, Keller M, Schwemmer F, Zehnle S, Mark D, von Stetten F, Zengerle R, Paust N
  (See online at https://doi.org/10.1039/C4CS00371C)
• Designer amphiphilic proteins as building blocks for the intracellular formation of organelle-like compartments. Nat Mater. 2015
  Huber MC, Schreiber A, von Olshausen P, Varga BR, Kretz O, Joch B, Barnert S, Schubert R, Eimer S, Kele P, Schiller SM
  (See online at https://doi.org/10.1038/NMAT4118)
• Fundamental properties of unperturbed haematopoiesis from stem cells in vivo. Nature. 2015
  Busch K, Klapproth K, Barile M, Flossdorf M, Holland-Letz T, Schlenner SM, Reth M, Höfer T, Rodewald HR
  (See online at https://doi.org/10.1038/nature14242)
• Lack of neuronal IFN-alpha-IFNAR causes lewy body- and parkinson's disease-like dementia. Cell. 2015
  Ejlerskov P, Hultberg JG, Wang J, Carlsson R, Ambjørn M, Kuss M, Liu Y, Porcu G, Kolkova K, Friis Rundsten C, Ruscher K, Pakkenberg B, Goldmann T, Loreth D, Prinz M, Rubinsztein DC, Issazadeh-Navikas S
  (See online at https://doi.org/10.1016/j.cell.2015.08.069)
• Mistargeted mitochondrial proteins activate a proteostatic response in the cytosol. Nature. 2015
  Wrobel L, Topf U, Bragoszewski P, Wiese S, Sztolsztener ME, Oeljeklaus S, Varabyova A, Lirski M, Chroscicki P, Mroczek S, Januszewicz E, Dziembowski A, Koblowska M, Warscheid B, Chacinska A
  (See online at https://doi.org/10.1038/nature14951)
• Molecular architecture of the active mitochondrial protein gate. Science. 2015
  Shiota T, Imai K, Qiu J, Hewitt VL, Tan K, Shen HH, Sakiyama N, Fukasawa Y, Hayat S, Kamiya M, Elofsson A, Tomii K, Horton P, Wiedemann N, Pfanner N, Lithgow T, Endo T
  (See online at https://doi.org/10.1126/science.aac6428)
• Nanoclusters of the resting T cell antigen receptor (TCR) localize to non-raft domains. Biochim Biophys Acta. 2015
  Beck-García K, Beck-García E, Bohler S, Zorzin C, Sezgin E, Levental I, Alarcón B, Schamel WW
  (See online at https://doi.org/10.1016/j.bbamcr.2014.12.017)
• Optogenetic control of Signalling in mammalian cells. Biotechnol J. 2015
  Beyer HM, Naumann S, Weber W, Radziwill G
  (See online at https://doi.org/10.1002/biot.201400077)
• Responsiveness of B cells is regulated by the hinge region of IgD. Nat Immunol. 2015
  Übelhart R, Hug E, Bach MP, Wossning T, Dühren-von Minden M, Horn AH, Tsiantoulas D, Kometani K, Kurosaki T, Binder CJ, Sticht H, Nitschke L, Reth M, Jumaa H
  (See online at https://doi.org/10.1038/ni.3141)
• Signalling thresholds and negative B-cell selection in acute lymphoblastic leukaemia. Nature. 2015
  Chen Z, Shojaee S, Buchner M, Geng H, Lee JW, Klemm L, Titz B, Graeber TG, Park E, Tan YX, Satterthwaite A, Paietta E, Hunger SP, Willman CL, Melnick A, Loh ML, Jung JU, Coligan JE, Bolland S, Mak TW, Limnander A, Jumaa H, Reth M, Weiss A, Lowell CA, Müschen M
  (See online at https://doi.org/10.1038/nature14231)
• Structural biology. Mechanistic insight from the crystal structure of mitochondrial complex I. Science. 2015
  Zickermann V, Wirth C, Nasiri H, Siegmund K, Schwalbe H, Hunte C, Brandt U
  (See online at https://doi.org/10.1126/science.1259859)
• Surface imaging beyond the diffraction limit with optically trapped spheres. Nat Nanotechnol. 2015
  Friedrich L, Rohrbach A
  (See online at https://doi.org/10.1038/nnano.2015.202)
• The fusogenic lipid phosphatidic acid promotes the biogenesis of mitochondrial outer membrane protein Ugo1. J Cell Biol. 2015; 210(6):951-60
  Vögtle FN, Keller M, Taskin AA, Horvath SE, Guan XL, Prinz C, Opalińska M, Zorzin C, van der Laan M, Wenk MR, Schubert R, Wiedemann N, Holzer M, Meisinger C
  (See online at https://doi.org/10.1083/jcb.201506085)
• The octahaem MccA is a haem c–copper sulfite reductase. Nature. 2015
  Hermann B, Kern M, La Pietra L, Simon J, Einsle O
  (See online at https://doi.org/10.1038/nature14109)
• Tissue inhibitor of metalloproteinases (TIMP)-1 creates a premetastatic niche in the liver through SDF-1/CXCR4-dependent neutrophil recruitment in mice. Hepatology. 2015
  Seubert B, Grünwald B, Kobuch J, Cui H, Schelter F, Schaten S, Siveke JT, Lim NH, Nagase H, Simonavicius N, Heikenwalder M, Reinheckel T, Sleeman JP, Janssen KP, Knolle PA, Krüger A
  (See online at https://doi.org/10.1002/hep.27378)
• Caveolin-1 regulates TCR signal strength and regulatory T-cell differentiation into alloreactive T cells. Blood. 2016
  Schönle A, Hartl FA, Mentzel J, Nöltner T, Rauch KS, Prestipino A, Wohlfeil SA, Apostolova P, Hechinger AK, Melchinger W, Fehrenbach K, Guadamillas MC, Follo M, Prinz G, Ruess AK, Pfeifer D, del Pozo MA, Schmitt-Graeff A, Duyster J, Hippen KI, Blazar BR, Schachtrup K, Minguet S, Zeiser R
  (See online at https://doi.org/10.1182/blood-2015-09-672428)
• Central nervous system myeloid cells as drug targets: current status and translational challenges. Nat Rev Drug Discov. 2016
  Biber K, Möller T, Boddeke E, Prinz M
  (See online at https://doi.org/10.1038/nrd.2015.14)
• Engineering and characterization of fluorogenic glycine riboswitches. Nucleic Acids Res. 2016
  Ketterer S, Gladis L, Kozica A, Meier M
  (See online at https://doi.org/10.1093/nar/gkw465)
• Gab2 is essential for Bcr-Abl mediated leukemic transformation and hydronephrosis in a chronic myeloid leukemia mouse model. Leukemia 2016
  Halbach S, Köhler M, Uhl FM, Huber J, Zeiser R, Koschmieder S, Aumann K, Brummer T
  (See online at https://doi.org/10.1038/leu.2016.92)
• Inhibition of T cell receptor Signalling by cholesterol sulfate, a naturally occurring derivative of membrane cholesterol. Nat Immunol. 2016
  Wang F, Beck-García K, Zorzin C, Schamel WW, Davis MM
  (See online at https://doi.org/10.1038/ni.3462)
• Mitochondrial protein synthesis adapts to Influx of nuclear-encoded protein. Cell. 2016
  Richter-Dennerlein R, Oeljeklaus S, Lorenzi I, Ronsör C, Bareth B, Schendzielorz AB, Wang C, Warscheid B, Rehling P, Dennerlein S
  (See online at https://doi.org/10.1016/j.cell.2016.09.003)
• MOF acetyl transferase regulates transcription and respiration in mitochondria. Cell. 2016
  Chatterjee A, Seyfferth J, Lucci J, Gilsbach R, Preissl S, Böttinger L, Mårtensson CU, Panhale A, Stehle T, Kretz O, Sahyoun AH, Avilov S, Eimer S, Hein L, Pfanner N, Becker T, Akhtar A
  (See online at https://doi.org/10.1016/j.cell.2016.09.052)
• On-demand erythrocyte disposal and iron recycling requires transient macrophages in the liver. Nat Med. 2016
  Theurl I, Hilgendorf I, Nairz M, Tymoszuk P, Haschka D, Asshoff M, He S, Gerhardt LM, Holderried TA, Seifert M, Sopper S, Fenn AM, Anzai A, Rattik S, McAlpine C, Theurl M, Wieghofer P, Iwamoto Y, Weber GF, Harder NK, Chousterman BG, Arvedson TL, McKee M, Wang F, Lutz OM, Rezoagli E, Babitt JL, Berra L, Prinz M, Nahrendorf M, Weiss G, Weissleder R, Lin HY, Swirski FK
  (See online at https://doi.org/10.1038/nm.4146)
• One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy. Nat Methods. 2016
  Yofe I, Weill U, Meurer M, Chuartzman S, Zalckvar E, Goldman O, Ben-Dor S, Schütze C, Wiedemann N, Knop M, Khmelinskii A, Schuldiner M
  (See online at https://doi.org/10.1038/NMETH.3795)
• Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor. Nat Med. 2016
  Rothhammer V, Mascanfroni ID, Bunse L, Takenaka MC, Kenison JE, Mayo L, Chao CC, Patel B, Yan R, Blain M, Alvarez JI, Kébir H, Anandasabapathy N, Izquierdo G, Jung S, Obholzer N, Pochet N, Clish CB, Prinz M, Prat A, Antel J, Quintana FJ
  (See online at https://doi.org/10.1038/nm.4106)
• Upgrading biomaterials with synthetic biological modules for advanced medical applications. Adv Drug Deliv Rev. 2016
  Wagner HJ, Sprenger A, Rebmann B, Weber W
  (See online at https://doi.org/10.1016/j.addr.2016.05.004)
• A somatic mutation in erythro-myeloid progenitors causes neurodegenerative disease. Nature. 2017
  Mass E, Jacome-Galarza CE, Blank T, Lazarov T, Durham BH, Ozkaya N, Pastore A, Schwabenland M, Chung YR, Rosenblum MK, Prinz M, Abdel-Wahab O, Geissmann F
  (See online at https://doi.org/10.1038/nature23672)
• Caveolin-1-dependent nanoscale organisation of the BCR regulates B cell tolerance. Nat Immunol. 2017
  Minguet S, Kläsener K, Schaffer AM, Fiala GJ, Osteso-Ibánez T, Raute K, Navarro-Lérida I, Hartl FA, Seidl M, Reth M, Del Pozo MA
  (See online at https://doi.org/10.1038/ni.3813)
• Dynamic cooperative glycan assembly blocks the binding of bacterial lectins to epithelial cells. Angew Chem Int Ed Engl. 2017
  Machida T, Novoa A, Gillon É, Zheng S, Claudinon J, Eierhoff T, Imberty A, Römer W, Winssinger N
  (See online at https://doi.org/10.1002/anie.201700813)
• Epigenetic regulation of ZBTB18 promotes glioblastoma progression. Mol Cancer Res. 2017
  Fedele V, Dai F, Masilamani AP, Heiland DH, Kling E, Gätjens-Sanchez AM, Ferrarese R, Platania L, Soroush D, Kim H, Nelander S, Weyerbrock A, Prinz M, Califano A, Iavarone A, Bredel M, Carro MS
  (See online at https://doi.org/10.1158/1541-7786.MCR-16-0494)
• Evidence of renal angiomyolipoma neoplastic stem cells arising from renal epithelial cells. Nat Commun. 2017
  Filipa Gonçalves A, Adlesic M, Brandt S, Hejhal T, Harlander S, Sommer L, Shakhova O, Wild PJ, Frew IJ
  (See online at https://doi.org/10.1038/s41467-017-01514-3)
• Histopathological findings in brain tissue obtained during epilepsy surgery. N Engl J Med. 2017
  Blumcke I, Spreafico R, et al.
  (See online at https://doi.org/10.1056/NEJMoa1703784)
• Mitochondrial priming by CD28. Cell. 2017
  Klein Geltink RI, O'Sullivan D, Corrado M, Bremser A, Buck MD, Buescher JM, Firat E, Zhu X, Niedermann G, Caputa G, Kelly B, Warthorst U, Rensing-Ehl A, Kyle RL, Vandersarren L, Curtis JD, Patterson AE, Lawless S, Grzes K, Qiu J, Sanin DE, Kretz O, Huber TB, Janssens S, Lambrecht BN, Rambold AS, Pearce EJ, Pearce EL
  (See online at https://doi.org/10.1016/j.cell.2017.08.018)
• Neuroplastin and Basigin are essential auyiliary subunits of plasma membrane ca2+-ATPases and key regulators of ca2+ clearance. Neuron. 2017
  Schmidt N, Kollewe A, Constantin CE, Henrich S, Ritzau-Jost A, Bildl W, Saalbach A, Hallermann S, Kulik A, Fakler B, Schulte U
  (See online at https://doi.org/10.1016/j.neuron.2017.09.038)
• RETINOBLASTOMA RELATED1 mediates germline entry in Arabidopsis. Science. 2017
  Zhao X, Bramsiepe J, Van Durme M, Komaki S, Prusicki MA, Maruyama D, Forner J, Medzihradszky A, Wijnker E, Harashima H, Lu Y, Schmidt A, Guthörl D, Logroño RS, Guan Y, Pochon G, Grossniklaus U, Laux T, Higashiyama T, Lohmann JU, Nowack MK, Schnittger A
  (See online at https://doi.org/10.1126/science.aaf6532)
• The BTG2-PRMT1 module limits pre-B cell expansion by regulating the CDK4-Cyclin-D3 complex. Nat Immunol. 2017
  Dolezal E, Infantino S, Drepper F, Börsig T, Singh A, Wossning T, Fiala GJ, Minguet S, Warscheid B, Tarlinton DM, Jumaa H, Medgyesi D, Reth M
  (See online at https://doi.org/10.1038/ni.3774)
• The IgM receptor FcR limits tonic BCR Signalling by regulating expression of the IgM BCR. Nat Immunol. 2017
  Nguyen TT, Kläsener K, Zürn C, Castillo PA, Brust-Mascher I, Imai DM, Bevins CL, Reardon C, Reth M, Baumgarth N
  (See online at https://doi.org/10.1038/ni.3677)
• The structure of vanadium nitrogenase reveals an unusual bridging ligand. Nat Chem Biol. 2017
  Sippel D, Einsle O
  (See online at https://doi.org/10.1038/NCHEMBIO.2428)
• A bound reaction intermediate sheds light on the mechanism of nitrogenase. Science. 2018
  Sippel D, Rohde M, Netzer J, Trncik C, Gies J, Grunau K, Djurdjevic I, Decamps L, Andrade SLA, Einsle O
  (See online at https://doi.org/10.1126/science.aar2765)
• Biomembrane permeabilization: Statistics of individual leakage events harmonize the interpretation of vesicle leakage. ACS Nano. 2018
  Braun S, Pokorná Š, Šachl R, Hof M, Heerklotz H, Hoernke M
  (See online at https://doi.org/10.1021/acsnano.7b08184)
• Continuous Signalling of CD79b and CD19 is required for the fitness of Burkitt lymphoma B cells. EMBO J. 2018
  He X, Kläsener K, Iype JM, Becker M, Maity PC, Cavallari M, Nielsen PJ, Yang J, Reth M
  (See online at https://doi.org/10.15252/embj.201797980)
• Innate immune memory in the brain shapes neurological disease hallmarks. Nature. 2018
  Wendeln AC, Degenhardt K, Kaurani L, Gertig M, Ulas T, Jain G, Wagner J, Häsler LM, Wild K, Skodras A, Blank T, Staszewski O, Datta M, Centeno TP, Capece V, Islam MR, Kerimoglu C, Staufenbiel M, Schultze JL, Beyer M, Prinz M, Jucker M, Fischer A, Neher JJ
  (See online at https://doi.org/10.1038/s41586-018-0023-4)
• Membrane protein insertion through a mitochondrial beta-barrel gate. Science. 2018
  Höhr AIC, Lindau C, Wirth C, Qiu J, Stroud DA, Kutik S, Guiard B, Hunte C, Becker T, Pfanner N, Wiedemann N
  (See online at https://doi.org/10.1126/science.aah6834)
• Microglial control of astrocytes in response to microbial metabolites. Nature. 2018
  Rothhammer V, Borucki DM, Tjon EC, Takenaka MC, Chao CC, Ardura-Fabregat A, de Lima KA, Gutiérrez-Vázquez C, Hewson P, Staszewski O, Blain M, Healy L, Neziraj T, Borio M, Wheeler M, Dragin LL, Laplaud DA, Antel J, Alvarez JI, Prinz M, Quintana FJ
  (See online at https://doi.org/10.1038/s41586-018-0119-x)
• Neurons under T cell attack coordinate phagocyte-mediated synaptic stripping. Cell. 2018
  Di Liberto G, Pantelyushin S, Kreutzfeldt M, Page N, Musardo S, Coras R, Steinbach K, Vincenti I, Klimek B, Lingner T, Salinas G, Lin-Marq N, Staszewski O, Costa Jordão MJ, Wagner I, Egervari K, Mack M, Bellone C, Blümcke I, Prinz M, Pinschewer DD, Merkler D
  (See online at https://doi.org/10.1016/j.cell.2018.07.049)
• Structural basis of membrane protein chaperoning through the mitochondrial intermembrane space. Cell. 2018
  Weinhäupl K, Lindau C, Hessel A, Wang Y, Schütze C, Jores T, Melchionda L, Schönfisch B, Kalbacher H, Bersch B, Rapaport D, Brennich M, Lindorff-Larsen K, Wiedemann N, Schanda P
  (See online at https://doi.org/10.1016/j.cell.2018.10.039)
• Synthetic Biology makes polymer materials count. Adv Mater. 2018
  Beyer HM, Engesser R, Hörner M, Koschmieder J, Beyer P, Timmer J, Zurbriggen MD, Weber W
  (See online at https://doi.org/10.1002/adma.201800472)
• Synthetic biologyinspired design of signal-amplifying materials systems. Materials Today 2018
  Wagner HJ, Engesser R, Ermes K, Geraths C, Timmer J, Weber W
  (See online at https://doi.org/10.1016/j.dib.2018.05.074)
• The chara genome: Secondary complexity and implications for plant terrestrialization. Cell. 2018
  Nishiyama T, Sakayama H, et al.
  (See online at https://doi.org/10.1016/j.cell.2018.06.033)