Die Rolle des Glorin-Signalsystems in der Multizellulären Entwicklung Sozialer Amöben (SIGMOSA)

• Antragsteller: Professor Dr. Pierre Stallforth; Professor Dr. Thomas Winckler
• Fachliche Zuordnung: Stoffwechselphysiologie, Biochemie und Genetik der Mikroorganismen ; Parasitologie und Biologie der Erreger tropischer Infektionskrankheiten
• Förderung: Förderung von 2018 bis 2022
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 398377386

• Erstellungsjahr: 2022

Zusammenfassung der Projektergebnisse

In this project, we investigated the molecular basis of glorin signaling using a combination of proteomics, metabolomics, and chemical biology approaches. This signaling network enables a group of social amoebae to orchestrate multicellular aggregation. Except for the nature of the signal molecule virtually nothing is known about the components of this network including the receptor(s), degrading enzymes(s), or other potential interaction partners. Based on the combination of bioassay-guided fractionation and photoaffinity labeling we could identify candidate proteins that may be involved in the complex interaction network. Furthermore, we established a metabolomics platform that allows to monitor the metabolite production of amoebae under different conditions.

Projektbezogene Publikationen (Auswahl)

• The chemoattractant glorin is inactivated by ester cleavage during early multicellular development of Polysphondylium pallidum. ACS Chem. Biol. 15 (2018), 1506-1513
  Heinrich, D., Barnett, R., Tweedy, L., Insall, R., Stallforth, P, Winckler, T.
  
• Yellow polyketide pigment suppresses premature hatching in social amoeba. Proc. Natl. Acad. Sci. U. S. A. 119, (2022), e2116122119
  Günther, M., Reimer, C., Herbst, R., Kufs, J. E., Rautschek, J., Ueberschaar, N., Zhang, S., Peschel, G., Reimer, L., Regestein, L., Valiante, V., Hillmann, F., Stallforth P.