Illumination of the Structure and Function of PptA, a Novel Type of Polyphosphate Granule Attached Protein in Organelle-like Polyphosphatosomes of Ralstonia eutropha H16

• Applicant: Professor Dr. Dieter Jendrossek
• Subject Area: Metabolism, Biochemistry and Genetics of Microorganisms
• Term: from 2018 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 400441076

• Final Report Year: 2023

Final Report Abstract

The properties of the polyphosphate (polyP)- and glutamine synthetase (GlnA1)-associated protein PptA of Ralstonia eutropha were determined in vitro, in vivo including fluorescence-microscopical investigations of eyfp fusions with pptA, glnA1 and genes of various polyP-associated proteins. While PptA showed a clear effect on the subcellular positioning of GlnA1 and of polyP granules it did not detectably effect GlnA1 activity or growth of the cells in general. Analysis of polyP kinases of R. eutropha, A. tumefaciens and E. coli revealed that some polyP kinases not only act on nucleoside-phosphates but also phosphorylate thiaminphosphate yielding enzyme cofactors and/or alarmones (thiamin-diphosphate and thiamintriphosphate).

Publications

• Formation of an Organic–Inorganic Biopolymer: Polyhydroxybutyrate–Polyphosphate. Biomacromolecules, 20(9), 3253-3260.
  Hildenbrand, Jennie C.; Reinhardt, Simone & Jendrossek, Dieter
  
• A universal polyphosphate kinase: PPK2c of Ralstonia eutropha accepts purine and pyrimidine nucleotides including uridine diphosphate. Applied Microbiology and Biotechnology, 104(15), 6659-6667.
  Hildenbrand, Jennie C.; Teleki, Attila & Jendrossek, Dieter
  
• The Multiple Roles of Polyphosphate in <b><i>Ralstonia eutropha</i></b> and Other Bacteria. Microbial Physiology, 31(2), 163-177.
  Rosigkeit, Hanna; Kneißle, Lea; Obruča, Stanislav & Jendrossek, Dieter
  
• Update on Thiamine Triphosphorylated Derivatives and Metabolizing Enzymatic Complexes. Biomolecules, 11(11), 1645.
  Bettendorff, Lucien
  
• Polyphosphat — ein unterschätztes Molekül. BIOspektrum, 28(7), 691-693.
  Jendrossek, Dieter & Hildenbrand, Jennie C.
  
• Polyphosphate Kinases Phosphorylate Thiamine Phosphates. Microbial Physiology, 33(1), 1-11.
  Hildenbrand, Jennie C.; Sprenger, Georg A.; Teleki, Attila; Takors, Ralf & Jendrossek, Dieter