Post-transkriptionelle Regulation von slam in frühen Drosophila Embryonen
Zusammenfassung der Projektergebnisse
Posttranscriptional control of gene expression involves transport and localization of the mRNA in the cytoplasm of the cell as well as regulated translation. Prominent cases of such regulation include proteins of neuronic synapses, for example. We have studied the function of RNA localization and translation with respect to the gene slam, which is essential for cellularization and germ cell migration in Drosophila embryos. Slam mRNA and protein form a complex and colocalize at the basal cortical compartment during cellularization. As Slam protein is required for slam mRNA localization and proper mRNA localization, for full translation, we aim to dissect that intricate relationship of slam mRNA and protein. We have established the TRICK and SunTag systems for tracking the spatio-temporal pattern of localization and translation using separate tags for mRNA and protein. This has provided evidence that a specific region in the slam coding sequence contains non-coding information for delaying translation until the mRNA reaches its target site at the basal cortex. Using a combined cell culture and embryo assay, we mapped the region mediating protein localization at the basal cortex independent of its mRNA. Mapping of the noncoding cis elements for RNA localization and translation control will be a step towards identifying the trans factors that control these activities in future experiments.
Projektbezogene Publikationen (Auswahl)
-
Localization and translation control ofslamin Drosophila cellularization. Fly, 12(3-4), 191-198.
Yan, Shuling & Großhans, Jörg
