Cellular responses to heterologously expressed antibiotic gene clusters (B03)

• Subject Area: Medical Microbiology and Mycology, Hygiene, Molecular Infection Biology
• Term: from 2019 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 398967434

This project will address the cellular responses and adaptations of the common host strain Streptomyces coelicolor to heterologously expressed gene clusters. Surprisingly little is known about what actually happens in a heterologous strain during natural product biosynthesis, besides the occurrence or non-occurrence of the desired compound. It will be studied if the novobiocin and caprazamycin gene clusters respond to global regulators of the heterologous host, how biosynthetic routes offered by the host affect precursor supply, and how these precursors are finally incorporated into the desired compound.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 261: Cellular Mechanisms of Antibiotic Action and Production
• Applicant Institution: Eberhard Karls Universität Tübingen
• Project Heads: Privatdozent Dr. Bertolt Gust; Professor Dr. Leonard Kaysser, until 9/2020