PPR proteins as modulators of chloroplast translation and RNA stability during temperature acclimation (A07)

• Subject Area: Plant Cell and Developmental Biology
• Term: from 2020 to 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 270050988

The PPR proteins SVR7 and PGR3 are important ribosome biogenesis factors and MRL1 is a PPR required for Rubisco expression. We found that all three PPR genes are induced during cold acclimation and that in general, ribosome biogenesis factors and ribosomal components are cold-regulated. Our goal is to understand how the selected PPR proteins influence RNA stability and translation of their target mRNAs during temperature acclimation. Furthermore, we will investigate by super resolution microscopy, where ribosome biogenesis takes place in chloroplasts and will use quantitative biochemistry to determine how ribosome numbers change in response to temperature acclimation.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 175: The Green Hub: Central Coordinator of Acclimation in Plants
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Dr. Hannes Ruwe