Project Details
Projekt
Molecular function of uracil phosphoribosyltransferase (UPP) in chloroplast proteostasis and its effect on acclimation (B08)
Subject Area
Plant Physiology
Term
since 2020
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 270050988
UPP is active in the salvage pathway and exhibits a moonlighting activity in chloroplast biogenesis. The project focuses on the identification of the molecular function of UPP in chloroplast proteostasis by testing already identified interaction partners (like PetC) by complementation approaches and direct assays (BIFC, CoIP). The entire UPP interactome will be identified through the combination of TurboID, Complexome analysis, forward genetics and the pamiR approach, in both Arabidopsis and Chlamydomonas, complemented by proteome studies of de-etiolation and light series experiments. Moreover, co-crystallization of UPP with interacting proteins is planned.
DFG Programme
CRC/Transregios
Applicant Institution
Ludwig-Maximilians-Universität München
Project Head
Dr. Torsten Möhlmann
