Project Details
Projekt
Dissection and correction of human regulatory T cell dysfunctions by CRISPR engineering (C04)
Subject Area
Immunology
Term
since 2021
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 452881907
Human regulatory T cells (Tregs) are attractive tools for cell therapies against autoimmunity. Here, we will employ CRISPR engineering to dissect transcriptional networks controlling Treg homeostasis and to revert Treg deficiencies. Using a pooled CRISPR screen in humanized mouse models, we will assess the influence of in vitro validated transcription factors with a special focus on SATB1 and HIVEP2 in human Treg development and function. In parallel, we will evaluate innovative non-viral genome targeting approaches for the correction of human Treg dysfunctions in humanized mouse models, using monogenic and life-threatening CARMIL2 deficiency as proof-of-principle.
DFG Programme
CRC/Transregios
Applicant Institution
Technische Universität München (TUM)
Project Heads
Dr. Daniel Kotlarz; Professorin Dr. Kathrin Schumann
