The biological relevance of NAD+-capping of RNA (A02)

• Subject Area: Biochemistry
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

Non-canonical RNA caps like NAD exist in both prokaryotes and eukaryotes, but their broader biological roles remain unclear. To address this issue, we will: (1) enhance the HELIOS NAD-seq platform for dynamic profiling of NAD-capped RNAs; (2) examine the processing of the NAD transcriptome in human cells using pharmacological and genetic tools; and (3) develop visualization methods employing aptamers and NAD-binding proteins to study subcellular localization. These efforts will elucidate NAD-RNA dynamics and the role of NAD capping in RNA modification and processing across various models.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Head: Professor Dr. Andres Jäschke