Novel modification mapping techniques and their application to single cell epitranscriptomics (C01)

• Subject Area: Bioinformatics and Theoretical Biology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

We will explore and develop different options to implement epitranscriptomics on the single-cell level. Both, established as well as newly developed RNA modification mapping techniques will be evaluated for their biochemical compatibility to be applied to a specific variant of single-cell RNA sequencing. Adaptations to single-cell mode will be implemented for a maximum of different modifications and RNA species, with the aim to establish an epitranscriptomic atlas for vertebrate embryogenesis, using zebrafish embryos as model.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Heads: Professor Dr. Mark Helm; Professor Dr. Andreas Hildebrandt, until 12/2025; Professorin Dr. Lauren Saunders; Professor Dr. Bertil Schmidt, until 12/2025