Project Details
Projekt
Mechanism of bacterial transenvelope protein and lipid transport (P02)
Subject Area
Biophysics
Term
since 2022
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 450648163
In project P02, we will use in situ pulsed electron spin resonance (ESR) spectroscopy combined with other biophysical approaches to elucidate the structural and dynamical basis for outer membrane biogenesis in Gram-negative bacteria. The substrate-dependent conformational rearrangements of the major periplasmic chaperon SurA and its interaction with the β-barrel assembly machinery (BAM) complex will be investigated, as well as the conformational dynamics/heterogeneity of the lipopolysaccharide (LPS) translocon LptDE structure in native membrane environment and in the presence of LPS or the periplasmic LPS-binding protein LptA. In the same way, the binding and inhibition mode for the peptidomimetic antibiotic thanatin on LptDE will be examined before we ultimately develop new ESR approaches to study heterooligomeric OMP complexes in native membranes.
DFG Programme
Collaborative Research Centres
Applicant Institution
Goethe-Universität Frankfurt am Main
Project Head
Dr. Benesh Joseph, Ph.D.
