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Projekt
Selenoprotein biosynthesis in Archaea (Selenoprotein Biosynthese in Archaea)
Antragsteller
Professor Dr. August Böck
Fachliche Zuordnung
Biochemie
Förderung
Förderung von 2000 bis 2003
Projektkennung
Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5241564
Archaeal selenocysteine insertion into polypeptides is directed by an mRNA element (SECIS) located in the 3' non-translated region and thus can be studied as a model for the eukaryal system. It has been discovered recently that the function of the bacterial SelB in selenoprotein synthesis is distributed over at least two proteins in an archaeon, one binding to the SECIS and the other one functioning as the specialized translation factor. This proposal aims at (i) the purification of the SECIS binding protein, (ii) the study of its interaction with the SECIS and the specialized translation factor, (iii) the construction of knock-out mutants for these genes and correlating thus the biochemical results with the in vivo function, and (iv) the mapping of the transcripts of an archaeal operon coding for four selenoproteins. The results obtained are to shed light on the mechanism of 3'-SECIS directed selenocysteine insertion.
DFG-Verfahren
Schwerpunktprogramme
