SFB 578:  Development of Biotechnological Processes by Integrating Genetic and Engineering Methods - From Gene to Product

• Subject Area: Thermal Engineering/Process Engineering ; Biology ; Medicine
• Term: from 2001 to 2012
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5484689

The collaborative research centre 578 aims at combining and integrating methods available both from the basic sciences and from the engineering sciences, notably genetics and biochemical engineering, to obtain high value-added products. The work primarily concerns processes for the microbial production of new heterologous recombinant proteins. The products to be focused on are either pharmaceutically active (bone morphogenetic proteins) or serve as biocatalysts to synthesize new oligosaccharides. The bacteria Escherichia coli (gram-negative) and Bacillus megaterium (gram-positive) as well as the mycelium-forming fungus Aspergillus niger will be used as host organisms. The aim of the research project basically is to investigate and understand the interaction of biological, biochemical, and engineering factors, aspects, and processes, taking the mentioned products and organisms as examples. The central aspects concern modelling of the biological system, product formation in the reactor, and product isolation and purification.

• DFG Programme: Collaborative Research Centres

Completed projects

• A01 - Production of recombinant glycosyltransferases using Bacillus megaterium and Aspergillus niger (Project Heads Dersch, Petra ;  Jahn, Dieter )
• A2 - Charakterisierung von Glycosyltransferasen des Glucansucrasetyps und Modifizierung ihrer Substratspezifität (Project Head Hofer, Bernd )
• A03 - Erzeugung, Screening und Charakterisierung modifizierter Glycosyltransferasen (Project Heads Buchholz, Klaus ;  Hofer, Bernd )
• A4 - Heterologe Expression von humanen Bone Morphogenetic Proteins in Escherichia coli (Project Head Gross, Gerhard )
• A5 - Analyse und Modellierung intrazellulärer Energie-, Stoff- und Informationsflüsse für die Proteinbiosynthese (Project Head Deckwer, Wolf-Dieter )
• A06 - Systems biology of chaperones for the production of antibodies with Bacillus megaterium (Project Heads Dübel, Stefan ;  Hust, Michael )
• A07 - Structual biology of glycosyltransferases for the optimisation of biotechnical processes (Project Heads Heinz, Dirk ;  Seibel, Jürgen )
• B1 - Heterologe Proteinproduktion mittels rekombinanter Mikroorganismen (Project Head Rinas, Ursula )
• B02 - Transport- und Reaktionsvorgänge in Biopellets (Project Heads Hempel, Dietmar C. ;  Horn, Harald )
• B03 - Influence of the environome on the morphology and productivity of filamentous fungi (Aspergillus niger) (Project Heads Hempel, Dietmar C. ;  Krull, Rainer )
• B04 - Systems Biology of Product Formation by Aspergillus niger (Project Heads Jahn, Dieter ;  Jänsch, Lothar ;  Nörtemann, Bernd )
• B05 - Systembiologie der Produktbildung bei Bakterien (Project Heads Deckwer, Wolf-Dieter ;  Franco-Lara, Ezequiel ;  Hempel, Dietmar C. ;  Jahn, Dieter )
• B06 - Bioinformatik metabolischer und regulatorischer Netzwerke (Project Head Zeng, An-Ping )
• B07 - Micromechanical properties of filamentous organims (Aspergillus niger) (Project Heads Kampen, Ingo ;  Kwade, Arno )
• B08 - Metabolic process analysis of Antibodies producing Bacillus megaterium (Project Heads Franco-Lara, Ezequiel ;  Hust, Michael ;  Krull, Rainer )
• B09 - Integrated databases, bioinformatic tools, analysis and modelling for systems biology with B. megaterium and A. niger (Project Heads Münch, Richard ;  Schomburg, Dietmar )
• B10 - Systems biotechnology of glycosytransferase production with Bacillus megaterium (Project Heads Franco-Lara, Ezequiel ;  Jahn, Dieter ;  Schomburg, Dietmar )
• B11 - Metabolic network dynamics for production of recombinant glycosyltransferases (Project Head Wittmann, Christoph )
• C01 - Aufarbeitung rekombinatner Proteine (Project Heads Deckwer, Wolf-Dieter ;  Schwedes, Jörg )
• C02 - Continuous chromatographic separation of ternary and quasi ternary mixtures (Project Head Seidel-Morgenstern, Andreas )
• C03 - Integrierte Produktaufbereitung in einem Mehrphasenreaktor durch selektive Adsorption (Project Heads Bohnet, Matthias ;  Buchholz, Klaus ;  Jördening, Hans Joachim )
• C4 - On-line Bioprozeßanalytik mit mikrostrukturierten Analysensystemen (Project Heads Bilitewski, Ursula ;  Büttgenbach, Stephanus )
• C06 - Nanoanalytics for protein production processes (Project Heads Ludwig, Frank ;  Schilling, Meinhard )
• C07 - Protein purification with functionalized magnetic nanoparticles (Project Heads Garnweitner, Georg ;  Schilling, Meinhard )
• D01 - Drug Delivery Systems for controlled protein release (Project Heads Bunjes, Heike ;  Menzel, Henning )
• D02 - Microchips for protein analytic and diagnostic (Project Heads Büttgenbach, Stephanus ;  Dübel, Stefan )
• T02 - Rekombinante Produktion neuartiger Phytasen mit Bacillus megaterium (Project Head Jahn, Dieter )
• Z - Central office (Project Head Jahn, Dieter )

• Applicant Institution: Technische Universität Braunschweig
• Participating University: Otto-von-Guericke-Universität Magdeburg
• Participating Institution: Helmholtz-Zentrum für Infektionsforschung (HZI)
• Spokesperson: Professor Dr. Dieter Jahn