Project Details
Projekt
Precision Nanopore sequencing of RNA modifications and processing events (C04*)
Subject Area
Biochemistry
Term
since 2026
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440
Nanopore direct RNA sequencing (DRS) can detect RNA modifications on single molecules but faces challenges in precision, recall, and sequencing structured RNAs. Current protocols are limited to polyadenylated RNAs, and tailored strategies for selective sequencing are lacking. The project C04 aims to develop targeted DRS approaches and improve protocols to enhance sequencing accuracy and RNA modification detection. We will establish RNA standards, quality control measures, and realistic benchmarks to improve software performance assessment. Community engagement through RMaP Challenges and hackathons will support advancements in RNA modification detection.
DFG Programme
CRC/Transregios
Subproject of
TRR 319:
RMaP: RNA Modification and Processing
Applicant Institution
Johannes Gutenberg-Universität Mainz
